地喹氯铵对脑胶质瘤细胞凋亡的影响

发布时间：2018-01-18 11:30

本文关键词：地喹氯铵对脑胶质瘤细胞凋亡的影响　出处：《中国老年学杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的探讨地喹氯铵对脑胶质瘤细胞增殖、凋亡及侵袭的影响。方法用50、100、200μmol/L的地喹氯铵作用于脑胶质瘤细胞U251、U87、C6,作用时间分别为12、24、48、72、96 h,MTT检测细胞增殖情况。流式细胞仪检测76μmol/L的地喹氯铵作用48 h后的脑胶质瘤细胞U251的细胞凋亡情况。Transwell小室检测地喹氯铵对脑胶质瘤细胞侵袭能力的影响。Western印迹检测细胞中PI3K、p-PI3K、Akt、p-Akt蛋白的表达水平。结果 50、100、200μmol/L的地喹氯铵对U251、U87、C6脑胶质瘤细胞增殖均具有抑制作用。地喹氯铵对脑胶质瘤细胞的增殖抑制作用随着作用时间的增加而增加,随着药物作用浓度的增加而增加。地喹氯铵作用胶质瘤U251、U87、C6细胞48 h的半数抑制浓度由大到小依次为:C6U87U251。76μmol/L的地喹氯铵作用后的脑胶质瘤细胞凋亡率明显高于对照组(P0.01)。脑胶质瘤细胞经地喹氯铵作用后的侵袭细胞数明显低于对照组(P0.01)。地喹氯铵作用后的脑胶质瘤细胞中PI3K、Akt的表达水平与对照组相比无明显差异,而p-PI3K、p-Akt的表达水平明显下降。结论地喹氯铵对脑胶质瘤细胞增殖、侵袭能力具有抑制作用,对脑胶质瘤细胞凋亡具有促进作用,作用机制与PI3K/Akt信号通路有关。
[Abstract]:Objective to investigate the effects of diammonium chloride on the proliferation, apoptosis and invasion of glioma cells. Methods 50,100,200 渭 mol/L of diammonium chloride was used to treat the glioma cell line U251 U87. The action time of C _ 6 was 12 ~ (24) C _ (24) O _ (48) C _ (2) H _ (72) ~ (96) h. MTT was used to detect cell proliferation. Flow cytometry was used to detect the action of dilqualkonium chloride at 76 渭 mol/L. Apoptosis of human glioma cell U251 after h .Transwell chamber assay of the effect of diammonium chloride on invasion of glioma cells. Western blot analysis of PI3 in human glioma cells. K. Results the expression level of p-PI3KCX AktPI3KGK and p-Akt protein was 50,100,200 渭 mol/L, and the expression level of p-PI3KT was 50,100,200 渭 mol/L on U251 U87. The proliferation of C6 glioma cells was inhibited, and the inhibitory effect of dilqualkonium chloride on the proliferation of glioma cells increased with the increase of the time of action. With the increase of drug action concentration, the glioma U251 U87 was induced by dilaquinamide. The apoptosis rate of glioma cells was significantly higher than that of the control group (P < 0.05). The half inhibitory concentration of C6 cells was higher than that of the control group (P < 0.05). The apoptosis rate of glioma cells was significantly higher than that of the control group (P < 0.01). The number of invasive cells in glioma cells treated with diqualone was significantly lower than that in the control group (P 0.01). The number of PI3K in glioma cells treated with diquinolium chloride was significantly lower than that in the control group. The expression level of Akt was not significantly different from that of the control group, but the expression level of p-PI3KN p-Akt was significantly decreased. Conclusion Diqualkonium chloride has an inhibitory effect on the proliferation and invasion of glioma cells. It can promote the apoptosis of glioma cells, and the mechanism is related to the PI3K/Akt signaling pathway.
【作者单位】：锦州医科大学药学院;锦州医科大学附属第一医院药品管理科;
【基金】：辽宁省自然科学基金(No.2014022044)
【分类号】：R96
【正文快照】： 目前常用的治疗脑胶质瘤的方法是手术治疗和放疗〔1〕,但由于脑胶质瘤具有发病隐匿、生长速度快、易转移、易复发等特点,手术治疗和放疗效果仍然不理想。地喹氯铵(DECA)是一种广谱抗菌药,对分枝杆菌、革兰阴性菌、革兰阳性菌等具有抑制作用〔2〕,对口腔溃疡、扁桃体炎、咽炎、

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