环磷酰胺联合柿叶乙酸乙酯部位对H22小鼠瘤组织抗氧化能力及Bcl-2,Bax蛋白表达的影响

发布时间：2018-01-19 18:23

本文关键词： 柿叶乙酸乙酯部位 H荷瘤小鼠抗氧化 B细胞淋巴瘤/白血病- Bcl-相关x蛋白　出处：《中国实验方剂学杂志》2015年15期 　论文类型：期刊论文

【摘要】：目的:通过研究环磷酰胺(CTX)联合柿叶乙酸乙酯部位(PE)对H22荷瘤小鼠瘤组织的抗氧化作用和对瘤组织凋亡相关蛋白B细胞淋巴瘤/白血病-2(Bcl-2)和Bcl-2相关x蛋白(Bax)蛋白表达的影响,探讨两药联合应用的效果。方法:昆明种小鼠,取H22荷瘤鼠腹水接种于昆明种小鼠右前肢腋窝皮下,建立H22荷瘤小鼠模型,将荷瘤小鼠随机分为模型组,CTX组(0.1 g·kg-1),CTX联合PE高、中、低剂量组(2,1,0.5 g·kg-1),连续ig给药10 d,处死小鼠后计算抑瘤率,采用金氏q值法判断两者合用的效果,检测瘤组织中超氧化物歧化酶(SOD)活力,丙二醛(MDA)含量,采用免疫组化法检测瘤组织中Bcl-2和Bax蛋白表达情况。结果:与模型组比较,各给药组抑瘤率明显升高,SOD水平和Bax蛋白表达均明显升高,MDA水平和Bcl-2蛋白表达明显降低(P0.05,P0.01);CTX联合PE各剂量组的抑瘤率均高于CTX组,0.85q1.15,两药合用作用相加;与CTX组比较,联合用药各剂量组SOD水平和Bax蛋白表达均升高,MDA水平,Bcl-2蛋白表达及Bcl-2/Bax均降低(P0.05,P0.01)。结论:CTX联合PE能使抑瘤作用相加,其机制可能与两药合用能明显增强H22小鼠机体抗氧化能力,下调Bcl-2蛋白及上调Bax蛋白的表达有关。
[Abstract]:Objective: to study the effect of cyclophosphamide (CTX) combined with ethyl acetate on persimmon leaves. Antioxidant effect on tumor tissue of H22 tumor-bearing mice and expression of apoptosis-related protein B cell lymphoma / leukemia-2Bcl-2) and Bcl-2 associated x protein Bax). The effect of protein expression. Methods: the mice with H22 tumor bearing mice were inoculated with ascites in the hypodermic armpit of the right forelimb of Kunming mice to establish the model of H22 tumor bearing mice. Tumor-bearing mice were randomly divided into CTX group (0.1 g 路kg ~ (-1)) combined with PE (0.5 g 路kg ~ (-1)). Mice were sacrificed for 10 days, and the inhibitory rate was calculated. The combined effect was evaluated by Kim's Q value method, and the activity of superoxide dismutase (SOD) in tumor tissue was detected. The expression of Bcl-2 and Bax in tumor tissue was detected by immunohistochemical method. Results: compared with the model group, the tumor inhibition rate of each administration group was significantly higher than that of the model group. The level of SOD and the expression of Bax protein increased significantly, and the expression of Bcl-2 protein decreased significantly. The inhibitory rate of CTX combined with PE was higher than that of CTX group (0.85q1.15). Compared with CTX group, the level of SOD and the expression of Bax protein were increased in each dosage group of combined medication. The expression of Bcl-2 protein and Bcl-2/Bax were decreased (P0.05). Conclusion the anti-tumor effect can be increased by the combination of the two drugs, and the anti-oxidation ability of H22 mice may be enhanced by the combination of the two drugs. Down-regulation of Bcl-2 protein and up-regulation of Bax protein expression were related.
【作者单位】：广西科技大学;广西科技大学第二附属医院;
【基金】：广西医学科学实验中心开放基金专项项目(KFJJ2011-17)
【分类号】：R965
【正文快照】： 肝癌是我国常见的恶性肿瘤之一,发病人数约占全球的55%,年病死率仅次于肺癌,是全球癌症相关死亡的第三大原因[1]。肝癌起病隐匿,进展迅速,生存质量差,生存期短。随着天然植物药回归的热潮,绿色抗肝癌植物药的研究越来越受到人们的关注[2]。柿叶为柿树科柿树属植物柿Diospyros

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