盐蒿提取液抗食源性致病菌活性及其抑菌机理

发布时间：2018-01-21 06:01

  本文关键词： 盐蒿提取液 抑菌活性 抑菌机理　出处：《江苏农业学报》2017年04期 　论文类型：期刊论文

【摘要】：以5种常见的致病菌为试验对象,采用体外抑菌试验筛选出盐蒿提取液作用效果最好的致病菌,并通过研究盐蒿提取液对致病菌作用前后细菌培养液的OD260、电导率、碱性磷酸酶(AKP)活性和细胞超微结构的变化,初步探讨盐蒿提取液对单增李斯特菌(Listeria monocytogenes)的抑菌机制。结果显示,盐蒿提取液对5种致病菌均有抑菌活性,但对单增李斯特菌抑制效果最好,最小抑菌浓度(MIC)为0.062 5 mg/ml,对5种致病菌抑制效果表现为:单增李斯特菌金黄色葡萄球菌大肠杆菌肠炎沙门氏菌鲍氏志贺氏菌。单增李斯特菌经盐蒿提取液处理后,细菌培养液260 nm的吸光度、电导率和AKP活性均增大,表明盐蒿提取液可破坏单增李斯特菌细胞壁和细胞膜的结构,导致细胞通透性增加,进而使细胞内容物外泄,使细胞生长受到抑制,进而导致死亡。
[Abstract]:With five common pathogenic bacteria as the experimental object, the bacteria with the best effect of extract of Artemisia Salina were screened by in vitro bacteriostasis test. The changes of OD260, electrical conductivity, alkaline phosphatase (ALP) activity and ultrastructure of bacteria culture medium before and after the action of Artemisia Salina extract on pathogenic bacteria were studied. The antibacterial mechanism of Artemisia Salina extract against Listeria monocytogenes was studied. The results showed that the extracts of Artemisia Salina had antimicrobial activity against five pathogenic bacteria. But the inhibitory effect on Listeria monocytogenes was the best, the minimum inhibitory concentration was 0.062 5 mg/ml. The inhibitive effects on 5 pathogenic bacteria were as follows: Listeria monocytogenes Staphylococcus aureus Escherichia coli Shigella baumannii and Listeria monocytogenes treated with Artemisia Salina extract. The absorbance, conductivity and AKP activity of bacterial culture medium increased at 260nm, which indicated that the extract of Artemisia Salina could destroy the structure of cell wall and cell membrane of Listeria monocytogenes, resulting in the increase of cell permeability. In turn, the cell contents leak out, so that cell growth is inhibited, leading to death.
【作者单位】： 江苏省农业科学院农产品加工研究所;江苏海苑食品有限公司;
【基金】：2015年国家留学基金项目(201508320085) 2014年苏北科技发展计划(富民强县)项目(BN2014147)
【分类号】：R915
【正文快照】： 盐蒿(Artemisia halodendron)又名碱蓬、龙须菜,属一年生草本植物,是一种生长于盐碱地和海滨沙滩的抗逆性较强的盐生植物[1]。中国盐蒿资源丰富,共有盐蒿属植物21种和1个变种[2],江苏拥有中国最大的沿海滩涂湿地,野生盐蒿的平均产量为1.5 t/hm2,产量十分可观[3]。盐蒿含有很多，

本文编号：1450669