别嘌醇改善果糖诱导的大鼠高尿酸血症与调节肠道葡萄糖转运子表达的关系研究

发布时间：2018-01-22 04:06

本文关键词： 别嘌醇果糖尿酸高尿酸血症葡萄糖转运子葡萄糖转运子　出处：《中国药理学通报》2017年04期 　论文类型：期刊论文

【摘要】：目的观察别嘌醇和苯溴马隆对高果糖饮水诱导的高尿酸血症(hyperuricemia,HUA)大鼠血尿酸水平、肝脏黄嘌呤氧化酶活性以及肠道果糖转运子(glucose transporter,GLUT)2和5表达的影响。方法 Wistar大鼠连续饮用10%果糖水8周以制备高尿酸血症大鼠模型,其中从第5周开始分别给予大鼠灌胃5 mg·kg~(-1)别嘌醇或10 mg·kg~(-1)苯溴马隆,共4周。磷钨酸法检测大鼠血尿酸水平,比色法检测肝脏XOD活性,Western blot法和免疫组化染色法检测肠道GLUT2和GLUT5的表达。结果别嘌醇明显降低果糖诱导的HUA大鼠血尿酸水平(P0.01)和肝脏XOD活性(P0.01),减少了HUA大鼠肠道GLUT5的表达(P0.01),但对肠道GLUT2的表达无明显影响。与此同时,苯溴马隆也明显降低了果糖诱导的HUA大鼠血尿酸水平(P0.01),但对HUA大鼠肝脏XOD活性、肠道GLUT2和GLUT5的表达均无明显影响。结论别嘌醇可明显降低果糖诱导的HUA大鼠血尿酸水平,其机制与抑制肝脏XOD活性以减少尿酸产生,抑制GLUT5表达以减少肠道果糖转运吸收,最终减少果糖代谢产生尿酸相关。
[Abstract]:Objective to observe the level of serum uric acid in hyperuricemia rats induced by high fructose drinking water by allopurinol and benbromalone. Liver xanthine oxidase activity and intestinal fructose transporter glucose transporter. Methods the rat model of hyperuricemia was established by drinking 10% fructose water for 8 weeks. Rats were given 5 mg 路kg ~ (-1) allopurinol or 10 mg 路kg ~ (-1) benzbromarone for 4 weeks from the 5th week. The level of serum uric acid was measured by phosphotungstic acid method. Liver XOD activity was detected by colorimetric method. Western blot and immunohistochemical staining were used to detect the expression of GLUT2 and GLUT5 in intestinal tract. Results allopurinol significantly decreased the level of serum uric acid in HUA rats induced by fructose. (P 0.01) and liver XOD activity (P 0.01). The expression of GLUT5 in the intestine of HUA rats was decreased, but the expression of GLUT2 in the intestine was not significantly affected. Benzimarone also significantly decreased the serum uric acid level of fructose induced HUA rats (P0.01), but the liver XOD activity of HUA rats. Conclusion allopurinol can significantly reduce the level of serum uric acid in HUA rats induced by fructose, and inhibit the activity of XOD in liver to reduce uric acid production. Inhibition of GLUT5 expression in order to reduce intestinal fructose transport absorption, and ultimately reduce fructose metabolism related to uric acid production.
【作者单位】：重庆市天然药物研究重点实验室重庆工商大学环境与资源学院;重庆医科大学附属第一医院中西医结合科;
【基金】：国家自然科学基金资助项目(No 81503420) 重庆市教委科学技术研究项目(No KJ1400626) 重庆市科委前沿与应用基础研究项目(No cstc2016jcyja0475)
【分类号】：R965
【正文快照】： 随着人们生活水平的提高和饮食结构的改变,高尿酸血症(hyperuricemia,HUA)发病率明显升高[1]。HUA不仅可导致痛风,还与肥胖、代谢综合征、2型糖尿病、高血压、冠心病、肾病等密切相关[2-3],因此如何防治HUA已成为全社会关注的公共健康课题。近10年来有关高果糖饮食与HUA高发病

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