叶酸对PCPA抑郁小鼠外侧缰核β-CaMKⅡ水平的影响

发布时间：2018-01-23 02:14

本文关键词： 叶酸抑郁症 PCPA β-钙调蛋白激酶　出处：《天然产物研究与开发》2017年07期 　论文类型：期刊论文

【摘要】：为探究叶酸对PCPA抑郁症模型小鼠的干预作用及其对外侧缰核β-Ca MK II水平的影响,将昆明种小鼠40只随机分为三组:对照组、模型组、叶酸组,通过腹腔注射PCPA(100 mg/kg)每日1次,连续4 d构建抑郁症模型,旷场与悬尾实验观察小鼠行为变化,HPLC检测小鼠下丘脑5-HT含量,免疫组化测定小鼠外侧缰核β-Ca MK II表达水平。结果显示,模型组与对照组相比自主活动能力下降,悬尾不动时间延长(P0.05),而叶酸组与对照组相比上述实验结果无统计学差异(P0.05)。模型组与对照组相比下丘脑5-HT含量降低,外侧缰核β-Ca MK II表达水平下降(P0.05)。结果表明叶酸能显著提高下丘脑5-HT含量与外侧缰核β-Ca MK II水平,其抗抑郁作用机制可能与之有关。
[Abstract]:In order to investigate the effect of folic acid on PCPA depression model mice and the effect of folic acid on the level of 尾 -Ca MK II in lateral habenula, 40 Kunming mice were randomly divided into three groups: control group and model group. In folic acid group, the depression model was established by intraperitoneal injection of PCPA(100 mg / kg once a day for 4 days. The behavior of mice was observed by open-field and tail suspension experiments. The content of 5-HT in hypothalamus and the expression of 尾 -CaMKII in lateral habenular nucleus of mice were detected by HPLC and immunohistochemistry. The results showed that the ability of autonomous activity in model group was lower than that in control group. The immobility time of tail was prolonged (P0.05A), but there was no statistical difference between the folic acid group and the control group (P 0.05). The content of 5-HT in hypothalamus of model group was lower than that of control group. The expression level of 尾 -CaMK II in the lateral habenular nucleus decreased (P 0.05). The results showed that folic acid could significantly increase 5-HT content in the hypothalamus and 尾 -Ca MK II level in the lateral habenular nucleus. Its antidepressant mechanism may be related to it.
【作者单位】：华东师范大学生命科学学院;
【基金】：上海市科委科研基目(16DZ2348900) 国家大学生创新项目资助
【分类号】：R965
【正文快照】： 抑郁症属于一种临床常见综合征,全世界目前约2亿左右患者,在致残性疾病中位于榜首。抑郁症病因十分复杂,涉及到交感神经、内分泌和免疫系统等多个方面[1]。单胺类神经递质假说认为抑郁症的生物学基础是5-羟色胺(5-HT)和/或去甲肾上腺素(NE)的利用缺陷。目前,许多治疗抑郁症的

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