番茄皂苷毒性实验研究

发布时间：2018-01-23 11:11

本文关键词： 番茄皂苷急性毒性亚急性毒性小鼠　出处：《食品科技》2017年11期 　论文类型：期刊论文

【摘要】：目的:研究番茄皂苷对小鼠急性毒性以及亚急性毒性的影响,为番茄皂苷的安全性作出初步评价。方法:急性毒性实验中,以剂量1.25、2.50、5.00 g/kg对小鼠一次性灌胃给药,给药后连续观察7 d,按时记录小鼠饮食饮水量和小鼠最终死亡率。观察结束后,将小鼠解剖,取心、肝、脾、肾进行肉眼观察,计算脏器系数,大致确定毒性靶器官。亚急性毒性实验中,以剂量0.50、2.00、5.00 g/kg对小鼠灌胃给药,连续给药30 d后,进行常规的血液生化指标测定并计算主要脏器系数以及对肝脏进行组织病理学检测。结果:急性毒性实验中,3组给药小鼠未见明显毒性反应,饮食饮水量与空白组比较均没有显著性差异;整个实验过程中,小鼠无1例死亡,番茄皂苷的致死量大于5.00 g/kg;雄性高剂量组小鼠的肝脏指数与空白组比较明显增大,有统计学意义,其余脏器系数与空白组对照未见明显异常。亚急性毒性实验中,除了雄性高剂量组小鼠的肝脏系数与空白组比较存在显著性差异,其余脏器系数均无统计学意义;血液生化指标测试中,雄、雌性3组给药组的血尿素氮的含量与空白组比较均降低,有统计学意义,其他指标未见异常;肝脏病理切片结果显示,各给药组均没有明显的肝损伤作用。结论:番茄皂苷安全无毒,可放心使用。
[Abstract]:Objective: to study the effects of tomato saponins on acute toxicity and subacute toxicity in mice and to evaluate the safety of tomato saponins. The mice were given 5. 00 g / kg intragastric administration for 7 consecutive days. The mice diet and the final mortality were recorded on time. After the observation, the mice were dissected, heart, liver and spleen were taken. Kidney was observed with naked eye, organ coefficient was calculated, and toxic target organ was roughly determined. In subacute toxicity experiment, mice were given intragastric administration with dose of 0.50 ~ 2.00 ~ 5.00 g / kg. After 30 days of continuous administration, routine blood biochemical indexes were measured, the main organ coefficients were calculated and the liver histopathology was detected. Results: in the acute toxicity test. There was no obvious toxic reaction in the three groups, and there was no significant difference between the diet and the blank group. During the whole experiment, there was no death in mice. The lethal dose of tomato saponins was more than 5.00 g / kg. Compared with the blank group, the liver index of the male high-dose group was significantly increased, and the other organ coefficients were not significantly abnormal compared with the blank group. Subacute toxicity test. There was no significant difference in liver coefficient between male high dose group and blank group, but there was no significant difference in other organ coefficients. In the blood biochemical index test, the blood urea nitrogen content in the male and female groups was lower than that in the blank group, and the other indexes were not abnormal. The pathological sections of liver showed that there was no obvious liver injury in each administration group. Conclusion: tomato saponins are safe and nontoxic and can be safely used.
【作者单位】：广西植物功能物质研究与利用重点实验室广西壮族自治区中国科学院广西植物研究所;广西中医药大学;
【基金】：广西科技创新能力与条件建设计划项目(桂科能1598025-32) 广西壮族自治区主席科技基金项目(16449-08) 广西自然科学基金项目(2015GSNSFBA139120) 广西植物功能物质研究与利用重点实验室主任基金项目(ZRJJ2014-5,ZRJJ2016-17)
【分类号】：R99
【正文快照】： 番茄是我们日常食用最多的一年生蔬菜之一,其果实营养丰富,具有特殊风味,可以生食、煮食、加工制成番茄酱、番茄汁等。一般认为番茄中主要的功效成分是番茄红素,其为胡萝卜素的一种。番茄红素是一种脂溶性的不饱和碳氢化合物,在番茄中除了脂溶性化合物外尚存在有含量并不低的

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