蟾饲五谷虫抗菌肽分离及抑菌试验

发布时间：2018-01-23 21:04

本文关键词： 蟾饲五谷虫抗菌性铜绿假单胞菌甲型伤寒沙门菌肠炎沙门菌。　出处：《沈阳药科大学学报》2015年06期 　论文类型：期刊论文

【摘要】：目的分离蟾饲五谷虫中的抗菌肽成分,检测其对部分常见菌株的抑菌活性,为抗菌肽后续的大规模分离、提取与应用给予指导。方法利用磷酸、醋酸盐缓冲液及串联柱色谱技术分离提取抗菌肽,筛选包括枯草芽孢杆菌、苏云金芽孢杆菌、保加利亚苏云金芽孢杆菌、大肠杆菌、大肠杆菌EHEC、大肠杆菌ETEC、大肠杆菌EIEC、大肠杆菌EAEC、阴沟肠杆菌、单增李斯特菌、铜绿假单胞杆菌、肠炎沙门菌、甲型伤寒沙门氏菌、沙门菌和金黄色葡萄球菌共15株细菌作为候选菌株,采用纸片抑菌圈法测定所得抗菌肽的抑菌活性。结果无论采用磷酸盐还是醋酸盐缓冲液提取所得的抗菌肽,对铜绿假单胞菌、甲型伤寒沙门菌和肠炎沙门菌都具有明显的抑制作用。结论蟾饲五谷虫体内所含抗菌肽具有一定的抗菌能力,但其抗菌肽的具体含量和组成还需进一步研究。
[Abstract]:Objective to isolate antimicrobial peptides from Xenopus vulgaris and to detect their antibacterial activity against some common strains, so as to provide guidance for large-scale isolation, extraction and application of antimicrobial peptides. Methods Phosphoric acid was used. Antimicrobial peptides were isolated and extracted by acetate buffer and tandem column chromatography, including Bacillus subtilis, Bacillus thuringiensis, Bacillus thuringiensis, Bacillus thuringiensis, Escherichia coli and Escherichia coli EHEC. E. coli ETEC, E. coli EIEC, E. coli EAEC, Enterobacter cloacae, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella enteritis, Salmonella typhimurium. There were 15 strains of Salmonella and Staphylococcus aureus as candidate strains. The antibacterial activity of the antimicrobial peptides was determined by disk inhibition circle method. Results the antimicrobial peptides extracted by phosphate or acetate buffer were used to treat Pseudomonas aeruginosa (Pseudomonas aeruginosa). Both Salmonella typhimurium A and Salmonella enteritidis have obvious inhibitory effects. Conclusion the antimicrobial peptides contained in Xenopus vulgaris have certain antimicrobial activity, but the specific content and composition of antimicrobial peptides need to be further studied.
【作者单位】：沈阳药科大学生命科学与生物制药学院;沈阳出入境检验检疫局;辽宁中医药大学第一临床学院;
【基金】：辽宁省教育厅基金资助项目(L2012362)
【分类号】：R915
【正文快照】： 蟾饲五谷虫是以蟾蜍科属中华大蟾蜍(Bufobufogar garizans Cantor)或黑眶蟾蛛(B.melanos-tictus Schneider)等尸体为培养基质,人工培养的丽蝇科昆虫大头金蝇或其近缘昆虫的干燥幼虫。主产于华中、华东地区,其他各地区均有分布,一年四季均可采收。蟾饲五谷虫蛋白质质量分数为62

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