氟西汀对慢性应激大鼠前额叶谷氨酸转运体GLT-1表达的影响

发布时间：2018-01-24 22:13

本文关键词： 慢性不可预见性应激谷氨酸转运体氟西汀前额叶免疫组织化学法蛋白印迹分析抑郁　出处：《中国药理学通报》2015年02期 　论文类型：期刊论文

【摘要】：目的研究氟西汀对慢性应激大鼠前额叶谷氨酸转运体1(GLT-1)的影响,进一步探讨氟西汀抗抑郁作用可能的分子机制。方法正常SD大鼠60只,随机分为对照组、慢性不可预见性应激(CUS)组和氟西汀组。对CUS组和氟西汀组大鼠进行CUS应激后,氟西汀组给予氟西汀治疗,对照组和CUS组给予生理盐水。实验结束后进行糖水偏好和旷场行为测试,并使用免疫组织化学法和蛋白印迹分析检测大鼠前额叶GLT-1的表达水平。结果 (1)行为学测试结果显示,CUS组大鼠糖水偏好、总行程、平均移动速度及直立次数均低于对照组(P0.01);氟西汀组上述指标均高于CUS组(P0.01)。(2)免疫组织化学法分析显示,CUS组与对照组比较,大鼠前额叶GLT-1表达下降(P0.01);经氟西汀治疗后,大鼠前额叶GLT-1表达比CUS组明显升高(P0.01)。(3)蛋白印迹分析显示,CUS组与对照组比较,大鼠前额叶GLT-1表达下降(P0.01);经氟西汀治疗后,大鼠前额叶GLT-1表达比CUS明显升高(P0.01)。结论慢性应激下调大鼠前额叶GLT-1表达水平,而氟西汀上调GLT-1表达水平,GLT-1表达增加可能是氟西汀抗抑郁作用的分子机制之一。
[Abstract]:Objective to study the effect of fluoxetine on the prefrontal glutamate transporter (GLT-1) in rats under chronic stress and to explore the possible molecular mechanism of antidepressant effect of fluoxetine. The rats in CUS group and fluoxetine group were treated with fluoxetine after CUS stress. The control group and CUS group were given normal saline. After the experiment, sugar water preference and open-field behavior were tested. Immunohistochemical method and Western blot analysis were used to detect the expression of GLT-1 in prefrontal lobe of rats. The average moving speed and the number of upright movement were lower than that of the control group (P 0.01). The above indexes in fluoxetine group were higher than those in CUS group (P 0.01). The immunohistochemical analysis showed that the CUS group was compared with the control group. The expression of GLT-1 in prefrontal lobe of rats was decreased (P 0.01). After fluoxetine treatment, the expression of GLT-1 in the prefrontal lobe of rats was significantly higher than that in the CUS group. Western blot analysis showed that the expression of GLT-1 in the prefrontal lobe of rats was higher than that in the control group. The expression of GLT-1 in prefrontal lobe of rats was decreased (P 0.01). The expression of GLT-1 in prefrontal lobe of rats was significantly higher than that of CUS after fluoxetine treatment. Conclusion chronic stress can down-regulate the expression of GLT-1 in prefrontal lobe of rats. But fluoxetine upregulated GLT-1 expression and GLT-1 expression may be one of the molecular mechanisms of fluoxetine antidepressant effect.
【作者单位】：武汉大学人民医院精神卫生中心;湖北大学教育学院心理学系;
【基金】：国家“十二五计划”项目课题(No 2012BAI01B05) 国家自然科学基金资助项目(No 30971040,81271496)
【分类号】：R965
【正文快照】： 0.01)。(3)蛋白印迹分析显示,CUS组与对照组比较,大鼠前额叶GLT-1表达下降(P0.01);经氟西汀治疗后,大鼠前额叶GLT-1表达比CUS明显升高(P0.01)。结论慢性应激下调大鼠前额叶GLT-1表达水平,而氟西汀上调GLT-1表达水平,GLT-1表达增加可能是氟西汀抗抑郁作用的分子机制之一。当

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