HPLC法测定注射用脱氧核苷酸钠的含量

发布时间：2018-02-01 20:19

本文关键词： 高效液相色谱法脱氧核苷酸钠含量测定　出处：《沈阳药科大学学报》2015年05期 　论文类型：期刊论文

【摘要】：目的建立高效液相色谱法测定注射用脱氧核苷酸钠的含量。方法采用Whatman TMPartisil-10 SAX(250mm×4.6mm,10μm)色谱柱,流动相为50mmol·L-1磷酸二氢钾溶液(取磷酸二氢钾6.80 g,加水800mL,用磷酸调节pH至3.3,加水稀释至1 000mL),柱温为30℃,检测波长为258 nm,流速为1.0mL·min-1。结果注射用脱氧核苷酸钠中脱氧核糖胞嘧啶核苷酸钠(d CMPNa)、脱氧核糖腺嘌呤核苷酸钠(d AMP-Na)、脱氧核糖胸腺嘧啶核苷酸钠(d TMP-Na)和脱氧核糖鸟嘌呤核苷酸钠(d GMP-Na)的质量浓度分别在0.900 0~90.30mg·L-1、0.940 0~94.70mg·L-1、0.880 0~87.60mg·L-1和0.740 0~74.00mg·L-1内与峰面积呈良好的线性关系,且r均大于0.999 5。4种脱氧核糖核苷酸钠的平均加样回收率分别为99.3%(RSD=1.2%)、99.1%(RSD=0.8%)、98.2%(RSD=1.1%)和100.1%(RSD=1.5%)(n=9)。结论该方法简便、专属,分离效果好,灵敏度高,可用于测定注射用脱氧核苷酸钠中4种脱氧核糖核苷酸钠的含量。
[Abstract]:Objective to establish the determination of Sodium Deoxyribonucleotide for Injection high performance liquid chromatography method. Using Whatman TMPartisil-10 SAX (250mm * 4.6mm, 10 m) column, the mobile phase was 50mmol - L-1 potassium dihydrogen phosphate (KH2PO4 6.80 g, water 800mL, adjusted to pH 3.3 with phosphoric acid, diluted with water to 1 000mL), the column temperature was 30 C, the detection wavelength was 258 nm, the flow rate was 1.0mL - min-1. results in Sodium Deoxyribonucleotide for Injection DNA cytosine nucleotide sodium (D CMPNa), DNA adenine nucleotide sodium (D AMP-Na), DNA thymine nucleotide sodium (D TMP-Na) and deoxyribose guanine nucleotide sodium (D GMP-Na) mass concentration respectively in 0.900 0~90.30mg. L-1,0.940 0~94.70mg - L-1,0.880 0~87.60mg - L-1 and 0.740 0~74.00mg - L-1 and peak area showed a good linear relationship, and the R was greater than 0.999 5.4 deoxyribonucleate sodium The average recovery rate was 99.3% (RSD=1.2%), 99.1% (RSD=0.8%), 98.2% (RSD=1.1%) and 100.1% (RSD=1.5%) (n=9) respectively. Conclusion the method is simple, exclusive, effective and sensitive, and can be used for the determination of 4 sodium deoxyribonucleotides in Sodium Deoxyribonucleotide for Injection.

【作者单位】：辽宁中医药大学附属第四医院药剂科;沈阳药科大学药学院;
【分类号】：R927.2;O657.72
【正文快照】： 基因以脱氧核糖核酸(deoxyribonucleic acid,DNA)的形式存在于染色体,是细胞内遗传物质的结构和功能单位[1]。DNA的基本单位是脱氧核苷酸,而脱氧核苷酸是由磷酸、脱氧核糖和含氮碱基组成,碱基共有腺嘌呤、鸟嘌呤、胞嘧啶和胸腺嘧啶4种,因此就构成4种不同的脱氧核苷酸,即脱氧胞

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