关于氯沙坦钾与血清蛋白、黄芩苷与乙酰胆碱酯酶相互作用及氮掺杂碳量子点的荧光特性

发布时间：2018-02-09 17:24

本文关键词： 荧光猝灭牛血清白蛋白乙酰胆碱酯酶氯沙坦钾芦丁黄芩苷氮掺杂碳量子点检测汞离子　出处：《河北大学》2017年硕士论文　论文类型：学位论文

【摘要】：本论文主要应用荧光光谱法和紫外-可见光谱法等多种方法探究了药物与蛋白质的相互作用和通过水热法合成氮掺杂的碳量子点实现了对Hg2+的测定。本论文分为四章。第一章:归纳概括了血清白蛋白、乙酰胆碱酯酶结构和功能,概述了用光谱法探究蛋白质与药物相互作用机理和内容。对碳量子点性质,合成方法及在有关领域的使用价值做了概括。第二章:用不同的光谱手段对氯沙坦钾与牛血清白蛋白间的相互作用进行探究,考察了黄酮化合物芦丁和黄芩苷存在下对其相互作用的干扰。氯沙坦钾可以明显地使牛血清白蛋白的荧光发生猝灭,证明氯沙坦钾对牛血清白蛋白的猝灭机理为静态猝灭过程。热力学参数ΔH(-134.3kJ·mol-1),ΔS(-368.4J.mol-1.K-1)和ΔG(-24.52kJ·mol-1~-20.83kJ·mol-1),表明氯沙坦钾与牛血清白蛋白之间的主要作用力为范德华力和氢键。此外,牛血清白蛋白与氯沙坦钾间的结合距离r为3.183nm,表明它们之间发生能量转移的可能性很大。当在芦丁和黄芩苷存在的条件下,牛血清白蛋白与氯沙坦钾的结合距离就变为3.225nm和3.494nm。此外牛血清白蛋白与氯沙坦钾的结合常数降低,说明氯沙坦钾、芦丁和黄芩苷在牛血清白蛋白上面存在竞争。最后,通过同步荧光光谱和三维光谱探究了药物与牛血清白蛋白作用前后,牛血清白蛋白结构的变化。第三章:利用荧光光谱法,紫外-可见吸收光谱法和圆二色谱法探究了黄芩苷与乙酰胆碱酯酶(AChE)的相互作用,得出了它们作用的机理,并对其结合常数、结合位点数以及结合距离作了计算,得出黄芩苷明显地猝灭了乙酰胆碱酯酶的内源性荧光,猝灭的机制主要是静态猝灭。热力学参数ΔH(-174.6kJ·mol-1),ΔS(-489.9 J·mol-1.K-1)和ΔG(-28.61kJ·mol-1~-23.71kJ·mol-1),说明黄芩苷与乙酰胆碱酯酶间的作用力是范德华力和氢键。此外,乙酰胆碱酯酶与黄芩苷的结合距离r为2.85nm,说明它们之间发生能量转移的几率很大。用同步荧光光谱,三维荧光光谱和圆二色谱探究了黄芩苷与乙酰胆碱酯酶作用前后,乙酰胆碱酯酶构象的变化。最后,对AChE的活性进行了研究,得出其IC50为124μM.第四章:通过水热法,以丝氨酸和乙二胺为原料合成了水溶性良好的氮掺杂的荧光碳量子点。在360nm的最佳激发波长下该氮掺杂碳量子点的量子产率为8.66%,用透射电镜、高分辨透射电镜、红外光谱和荧光光谱等方法对其进行了系统的分析表征,表明该氮掺杂的碳量子点表面上含有丰富的羟基和羧基等基团,因此其水溶性良好。通过实验表明,汞离子使该氮掺杂碳量子点的荧光强度明显降低,其线性范围为1-20μM,检出限为7.33×10-5 mol·L-1,说明氮掺杂碳量子点在离子检测方面具有潜在的应用价值。
[Abstract]:A variety of methods in this thesis using fluorescence spectroscopy and UV Vis spectroscopy to explore the interactions between drugs and proteins and by CQDS hydrothermal synthesis of nitrogen doped to achieve the measurement of Hg2+. This thesis is divided into four chapters. The first chapter: To summarize the serum albumin, structure of acetylcholinesterase and, the paper outlines the spectral method to explore the interactions between protein and drug content. The mechanism and properties of c-dots, synthesis method and use value in the relevant fields are summarized. The second chapter: the interaction between bovine serum albumin and losartan to explore the spectrum means different, effects of flavonoids rutin the presence of baicalin and its interaction interference. Losartan potassium can obviously cause the fluorescence quenching of bovine serum albumin, proved that the quenching mechanism of bovine serum albumin for losartan potassium A static quenching process. The thermodynamic parameters of H (-134.3kJ mol-1), S (-368.4J.mol-1.K-1) and G (-24.52kJ mol-1~-20.83kJ mol-1), showed that the main force between losartan and bovine serum albumin as van Edward and hydrogen bonding. In addition, bovine serum albumin and potassium chloride candesartan combination between the distance r is 3.183nm that suggests the possibility of energy transfer occurs between them. When in the presence of rutin and baicalin conditions, reduce the binding constant of bovine serum albumin with losartan potassium distance becomes 3.225nm and 3.494nm. addition of bovine serum albumin with losartan potassium, indicating losartan potassium, rutin and baicalin compete in bovine serum albumin above finally, the synchronous fluorescence and three-dimensional spectral to explore the effects of drugs with bovine serum albumin before and after the change of bovine serum albumin structure. The third chapter: by fluorescence spectroscopy, UV Vis absorption spectroscopy and circular two chromatography on baicalin and acetylcholinesterase (AChE) interaction, the mechanism of their action, and the binding constants, binding sites and binding distances were calculated, the baicalin obviously quenches the intrinsic fluorescence quenching of acetylcholinesterase. The main mechanism is static quenching. The thermodynamic parameters of H (-174.6kJ mol-1), S (-489.9 J mol-1.K-1) and G (-28.61kJ mol-1~-23.71kJ mol-1), shows that the force of baicalin and acetylcholinesterase among the van Edward force and hydrogen bond. In addition, acetylcholinesterase and baicalin combined with distance R 2.85nm instructions the energy transfer occurs between them a great chance. By synchronous fluorescence, three-dimensional fluorescence spectroscopy and circular chromatography on two baicalin and AChE activity before and after the changes of acetylcholine esterase. The conformation After the activity of AChE was studied, the IC50 124 M. fourth chapter: by hydrothermal method with serine and ethylenediamine as fluorescent carbon quantum dots synthesized water soluble nitrogen doped well. In quantum yield the optimum excitation wavelength of 360nm under the nitrogen doped carbon quantum dots is 8.66% and by transmission electron microscopy, high resolution transmission electron microscopy, infrared spectroscopy and fluorescence spectroscopy were used to characterize the system, indicates that the carbon quantum dots on the surface of the nitrogen doped rich hydroxyl and carboxyl groups, so the good water solubility. Experiments show that the fluorescence intensity of the mercury ion nitrogen doping carbon quantum dots decreased, the linear range of 1-20 M and a detection limit of 7.33 * 10-5 mol - L-1, it is indicated that nitrogen doped carbon quantum dots has potential applications in ion detection.

【学位授予单位】：河北大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R96;O657.3

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