同位素比质谱方法检测兴奋剂福美司坦

发布时间：2018-02-12 16:54

本文关键词： 福美司坦同位素比质谱兴奋剂　出处：《中国运动医学杂志》2015年06期 　论文类型：期刊论文

【摘要】：目的:建立对福美司坦药物来源的检测方法,并进行人体代谢的检测研究。方法:采用酶解、提取、高效液相色谱纯化等方法对尿样进行预处理,应用同位素比质谱仪测定相关组分的同位素比(13C/12C,δ值),建立检测方法。本实验中招募2名男性健康受试者,每位受试者口服福美司坦后收集尿样,应用所建立的检测方法对尿样进行分析,探讨福美司坦在人体内代谢过程中的检测规律。结果:建立的分析方法精密度及重现性RSD均小于1%。受试者尿样中福美斯坦的δ值在服药后迅速降低,并在27小时内保持在约为-31‰。内源性雄性激素代谢物雄酮androsterone和本胆烷醇酮etiocholanolone的δ值无显著性变化。结论:在兴奋剂检测中确定以福美司坦药物原形为目标化合物进行来源分析,建立了检测福美斯坦兴奋剂的同位素比质谱分析方法。
[Abstract]:Objective: to establish a method for the detection of the origin of Fumestan and to study the metabolism of human body. Methods: urine samples were pretreated by enzymolysis, extraction and purification by HPLC. The isotopic ratio of 13C / 12C, 未 value of the related components was determined by means of isotope mass spectrometer, and a method was established. Two healthy male subjects were recruited in this experiment, and each of them took Fumestan orally and collected urine samples. The urine samples were analyzed by the established method. Objective: to study the detection rule of Fumestan during metabolism in human body. Results: the precision and reproducibility of the method were less than 1. The 未 value of Fumestan in urine decreased rapidly after taking medicine. The 未 values of androsterone androsterone and etiocholanolone, the endogenous metabolites of androgen, did not change significantly within 27 hours. Conclusion: the target combination of Fumestan drug in stimulant detection is determined. For source analysis, A mass spectrometry method for the determination of fumestan stimulants was established.
【作者单位】：国家体育总局反兴奋剂中心;北京体育大学;北京中医药大学;
【基金】：国家体育总局重点研究领域攻关课题(编号:2013B003)
【分类号】：R927;O657.63

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