中国南海深海真菌Exophiala sp. SCSIO 05791次级代谢产物研究

发布时间：2018-02-14 19:35

本文关键词： 中国南海海洋真菌次级代谢产物外瓶霉属　出处：《天然产物研究与开发》2017年06期 　论文类型：期刊论文

【摘要】：对中国南海500 m深海水来源真菌Exophiala sp.SCSIO 05791进行了次级代谢产物研究;综合运用硅胶柱层析、Sephadex LH-20凝胶柱层析、中压反相柱层析以及半制备高效液相等色谱学方法对其大米发酵产物进行分离纯化,从中分离到8个单体化合物,利用核磁共振、质谱等波谱学手段并结合其理化性质及文献数据鉴定了其化学结构:stephacidin A(1)、吲哚-3-乙酸(2)、环(D)-脯氨酸-(D)-苯丙氨酸(3)、反式阿魏酸(4)、顺式阿魏酸(5)、2-(乙酰氨基)-苯酚(6)、氨基苯甲酸(7)、4-羟基苯酞(8)。化合物1~8均为首次从该属中分离得到。
[Abstract]:The secondary metabolites of the 500m deep-sea water source fungus Exophiala sp.SCSIO 05791 in the South China Sea were studied, and the silica gel column chromatography and Sephadex LH-20 gel column chromatography were used. The rice fermentation products were separated and purified by mid-pressure reversed phase column chromatography and semi-preparative high performance liquid chromatography. Eight monomers were isolated from the fermentation products and used nuclear magnetic resonance (NMR). The chemical structures of 1: Stephacidin A ~ (1), Indole ~ (3) -acetic acid ~ (2 +), D ~ (2 +) -proline (D ~ (2 +)) -D ~ (2 +) -phenylalanine ~ (3), trans-ferulic acid ~ (4), cis-ferulic acid (5 ~ (2)) (acetamino-phenol ~ (6)) have been identified by means of mass spectrometry, and their chemical structures have been identified by means of mass spectrometry and literature data. Compounds 1 and 8 were isolated from this genus for the first time.
【作者单位】：中国科学院南海海洋研究所广东省海洋药物重点实验室;中国科学院大学;南海生物资源开发与利用协同创新中心;
【基金】：国家自然科学基金(41476135)
【分类号】：R915

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