索拉胶对皮肤的保护作用研究

发布时间：2018-02-26 18:39

本文关键词： 皮肤索拉胶保湿防皱抗紫外伤口愈合　出处：《南京理工大学》2014年硕士论文　论文类型：学位论文

【摘要】：作为身体最大的器官,皮肤是身体抵御外界侵害的第一道防线,具有许多重要的作用。受到自身和外界环境的影响,皮肤逐渐出现干燥、发黄、松弛、暗淡、皱纹等现象。β-(1,3)-葡聚糖作为一种生物大分子,因具有高粘度、保湿、防皱、抗衰老和促进伤口愈合等生物学活性,而被应用于化妆品中,但由于β-(1,3)-葡聚糖的提取和纯化复杂,使得其价格昂贵,难以被广泛应用。因此,研究水溶性的胞外p-(1,3)-葡聚糖—索拉胶在保湿、防皱、抗紫外和伤口愈合方面的性能,对于更好的利用和发挥索拉胶在化妆品和伤口愈合中的作用有着重要的意义。在相对湿度为43%和81%的环境中,索拉胶的吸湿率分别为13.23%和20.07%；在相对湿度为43%的环境中,索拉胶的保湿率为23.93%,转移至硅胶密闭容器里12小时,索拉胶的保湿率为15.10%。人体皮肤保湿实验中,与处理前皮肤的含水率相比,索拉胶处理后的皮肤含水量增加百分比为82.05%,8小时后为43.58%。在成纤维细胞中,索拉胶加强了Collα1和Col3α1基因的表达。在紫外线UVA照射前、后用索拉胶处理成纤维细胞3小时,可以显著提高细胞的存活率,与照射组的细胞存活率67.93%相比,照射前、后处理索拉胶的细胞存活率为100.94%、97.88%。小鼠实验中,索拉胶显著降低了紫外照射引起的细胞因子IL-1β、TNF-α和IL-6的表达,提高了Collα1和Co13α1基因的表达,组织切片显示,索拉胶可以削弱紫外线对皮肤中胶原纤维的破坏作用,维持皮肤的正常形态。在体外,索拉胶可以有效清除超氧阴离子自由基、羟基自由基和DPPH自由基,其清除率分别为17.53%、60.33%和43.82%。在小鼠全皮层伤口模型中,索拉胶加速了伤口的愈合,第12天其伤口愈合率为5.38%,而对照组的为24.76%；提高了细胞因子IL-1p和TNF-α基因在伤口愈合前期的表达,缩短了其高表达的时间,减轻了IL-1β和TNF-α的持续高表达对伤口愈合的不利作用。索拉胶可以促进成纤维细胞的增殖,与对照组相比,索拉胶的浓度为500μg/ml时,其细胞增殖率为124.95%。所有研究表明,索拉胶具有良好的吸湿和保湿性能,可以提高皮肤含水率,并具有潜在的防皱作用,减轻紫外线对皮肤的伤害作用,有效清除自由基,促进伤口愈合,可应用于化妆品和伤口愈合类药物等领域。
[Abstract]:As the largest organ of the body, skin is the body's first line of defense against external aggression and has many important functions. Under the influence of itself and the external environment, the skin gradually appears dry, yellow, relaxed and dark. Wrinkle and other phenomena. As a kind of biological macromolecule, 尾 -Che _ 1O _ 3C _ (- glucan) has been used in cosmetics because of its high viscosity, moisturizing, anti-wrinkle, anti-aging and promoting wound healing, but its extraction and purification are complicated. Therefore, to study the properties of water-soluble extracellular p-P- (1 +) -dextran-Sorghan in moisturizing, anti-wrinkle, anti-ultraviolet, and wound healing properties, which make it expensive and difficult to be widely used. It is of great significance to better use and play the role of Solan in cosmetic and wound healing. In the environment with relative humidity of 43% and 81%, the hygroscopic rates of sorghum were 13.23% and 20.07, respectively, and in the environment of 43% relative humidity, the moisture retention rate of sorghum was 23.93 and transferred to the silica gel closed container for 12 hours. In the skin moisturizing experiment, compared with the moisture content of the skin before the treatment, the moisture content of the skin treated with the solan gel increased by 82.05% and 43.58% after 8 hours. In fibroblasts, Solactin enhanced the expression of Coll 伪 1 and Col3 伪 1 genes. The survival rate of fibroblasts treated with Sola gel for 3 hours after ultraviolet UVA irradiation was significantly increased compared with that of irradiation group (67.93%). The cell survival rate of post-treated solan was 100.94% and 97.88%, compared with that of irradiation group (67.93%). Sola significantly decreased the expression of IL-1 尾 -TNF- 伪 and IL-6, and increased the expression of Coll 伪 1 and Co13 伪 1 genes. In vitro, Sola gum could effectively scavenge superoxide anion radical, hydroxyl radical and DPPH radical, and the scavenging rates were 17.53% and 43.82%, respectively. The wound healing rate was 5.38 on the 12th day and 24.76 in the control group, which increased the expression of cytokines IL-1p and TNF- 伪 in the early stage of wound healing and shortened the time of high expression. The continuous high expression of IL-1 尾 and TNF- 伪 could promote the proliferation of fibroblasts. Compared with the control group, the proliferation rate was 124.95% at the concentration of 500 渭 g / ml of IL-1 尾 and TNF- 伪. All the studies have shown that Sola gum has good moisture absorption and moisture retention, can increase moisture content of skin, and has potential anti-wrinkle effect, reduces the harm of ultraviolet rays to the skin, effectively clears free radicals, and promotes wound healing. Can be used in cosmetics and wound healing drugs and other fields.
【学位授予单位】：南京理工大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R96

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