dl-扁桃酸对精子质膜及顶体的完整性和线粒体膜电位的影响

发布时间：2018-02-27 04:00

本文关键词： dl-扁桃酸精子质膜精子顶体线粒体膜电位　出处：《中国新药杂志》2015年05期 　论文类型：期刊论文

【摘要】：目的:研究dl-扁桃酸(dl-mandelic acid)的抑精作用及其机制。方法:dl-扁桃酸0.5~10.0g·L-1处理高活力精子20 s,计算机辅助精子分析仪(computer assistant spermanalysis,CASA)测定精子活率、精子直线运动速率(VSL)、曲线运动速率(VCL)、平均路径速率(VAP)、侧摆幅度(ALH)、精子运动向前性(STR)和精子运动直线性(LIN);流式细胞(SYBR-14/PI)检测精子质膜完整性,采用FITC标记的豌豆凝集素荧光染色观察精子顶体完整性;FITC-PSA/PI/JC-1荧光染色检测精子线粒体膜电位变化。结果:随着dl-扁桃酸浓度的升高,精子活率与精子直线运动速率(VSL)、曲线运动速率(VCL)、平均路径速率(VAP)、侧摆幅度(ALH)、精子运动向前性(STR)和精子运动直线性(LIN)显著降低,dl-扁桃酸抑制精子活率的半数有效浓度(EC50)约为1.25 g·L-1,最低有效浓度(MEC)约为2.5 g·L-1。流式细胞仪检测表明,dl-扁桃酸对精子质膜完整性无影响。荧光染色检测表明,dl-扁桃酸处理组精子顶体受损,精子线粒体膜电位降低。结论:dl-扁桃酸通过影响精子顶体和降低线粒体膜电位,对精子起到抑制作用。
[Abstract]:Objective: to study the effect of dlmandelic acid (dl-mandelic acid) on spermatogenesis and its mechanism. Methods: the sperm motility was measured by computer aided sperm analyzer (assistant) and the sperm motility was determined by the treatment of high motility sperm with 0.5% dlmandelic acid (0.5 渭 g 路L -1) for 20 s. Sperm linear motility rate (VSLA), curve motion rate (VCL), mean path rate (VAP), lateral swing amplitude (ALHN), sperm motility forward (STR) and sperm motility linear line (SPL), flow cytometry (SYBR-14 / Pi) were used to detect the integrity of sperm plasma membrane. FITC labeled pea lectin fluorescence staining was used to observe the sperm acrosomal integrity (FITC-PSAP / Pi / JC-1) fluorescence staining. Results: with the increase of dlmandelic acid concentration, the mitochondrial membrane potential of spermatozoa was detected by FITC-PSA-PI- / JC-1 fluorescence staining. Sperm motility and sperm linear motility rate (VSLL), curvilinear motility rate (VCLA), mean path rate (VAP), lateral swing amplitude (ALHN), sperm motility forward (STR) and sperm motility linear line (SLL) significantly decreased the median effective concentration of spermatozoa motility inhibited by mandelic acid. Flow cytometry showed that dlmandelic acid had no effect on the integrity of sperm plasma membrane. Fluorescence staining showed that sperm acrosome was damaged in dl- mandelic acid treatment group. Conclusion the spermatozoa can be inhibited by the effect of 1% dlmandelic acid on acrosome and mitochondrial membrane potential.
【作者单位】：上海市计划生育科学研究所国家计划生育药具重点实验室;复旦大学公共卫生学院;
【基金】：国家科技重大专项(2012ZX10001007-009-003,2013ZX10001006-003-002) 十二五国家科技支撑计划(2012BAI31B04) 国家自然科学基金青年基金项目(81100459) 上海市科委项目(13431900800) 上海市人口和计划生育委员会(2012JG03)
【分类号】：R96

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