脑室给予不同剂量的Capsazepine对LPS致大鼠发热过程的影响

发布时间：2018-03-01 07:16

本文关键词： 发热瞬时受体电位香草酸受体 Capsazepine LPS 下丘脑 Ca+　出处：《中国药理学通报》2015年01期 　论文类型：期刊论文

【摘要】：目的在大鼠侧脑室内给予不同剂量的瞬时受体电位香草酸受体1(transient receptor potential vanilloid 1,TRPV1)拮抗剂Capsazepine,观察对LPS致热过程的影响。方法在侧脑室内预先给与3种剂量Capsazepine,随之腹腔给与LPS致热。在腹腔内埋植发射子遥测体温变化过程,同时应用荧光测定法检测下丘脑细胞内钙离子浓度的变化,Western blot方法检测TRPV1表达水平。结果随着Capsazepine剂量增大,LPS致大鼠发热水平升高,下丘脑细胞内钙离子浓度减少,TRPV1表达水平降低。结论 Capsazepine作用于大鼠下丘脑,可使LPS诱导的发热水平提高,此效应可能与TRPV1的作用有关。
[Abstract]:Objective to observe the effect of capsazepine, a transient receptor potential vanilloid 1 TRPV1 antagonist, on the process of fever induced by LPS in rat lateral ventricle. Methods three doses of capsazepine were given in the lateral ventricle, followed by intraperitoneal administration of Capsazepine. LPS fever. Emitters embedded in the abdominal cavity telemetry body temperature change process, Meanwhile, the changes of intracellular calcium concentration in hypothalamus cells were detected by fluorescence assay and the expression of TRPV1 was detected by Western blot. Conclusion the effect of Capsazepine on hypothalamic hypothalamus can increase the level of fever induced by LPS, which may be related to the effect of TRPV1.
【作者单位】：中国医科大学基础医学院生理学教研室;
【基金】：国家自然科学基金主任基金(No 31340074) 辽宁省自然科学基金(No 201202277)
【分类号】：R96

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