沙利度胺乳膏治疗皮炎湿疹药效学及抗炎机制

发布时间：2018-03-02 03:15

本文关键词： 沙利度胺乳膏皮炎湿疹小鼠 TNF-α IL- IL- IL- IL-　出处：《中国医院药学杂志》2017年19期 　论文类型：期刊论文

【摘要】：目的:研究沙利度胺乳膏对小鼠皮炎湿疹模型的治疗作用及抗炎机制。方法:60只小鼠被随机分为正常对照组,模型对照组,沙利度胺乳膏低、中、高浓度组和阳性(氢化可的松)对照组(每组10只)。采用二硝基氯苯(DNCB)诱导小鼠慢性皮炎湿疹模型。正常对照组和模型对照组给予空白基质,其他用药组外用给药,1 d 2次,连续15 d。千分尺测量小鼠左右耳中部厚度,计算左右耳厚度差;电子天平称量左右耳片质量,计算左右耳质量差;HE染色观察小鼠耳组织病理学变化,并进行病理组织学评分;ELISA法测定小鼠血清中TNF-α、IL-2、IL-4、IL-12、IL-18的含量。结果:沙利度胺乳膏能减轻小鼠耳厚度,减轻耳部炎症程度,升高其降低的IL-2、IL-4、IL-18水平,降低其升高的TNF-α、IL-12水平,并呈现良好的量效关系。结论:沙利度胺乳膏对皮炎湿疹有较好的治疗作用,其作用机制可能与调节Th1和Th2平衡有关。沙利度胺乳膏有望开发成一种治疗慢性皮炎湿疹的新药。
[Abstract]:Objective: to study the therapeutic effect and anti-inflammatory mechanism of thalidomide cream on mice with dermatitis and eczema. Methods: 60 mice were randomly divided into normal control group, model control group, low and middle salidomide cream. The chronic dermatitis eczema model of mice was induced by DNCBs in high concentration group and hydrocortisone positive control group (10 rats in each group). Normal control group and model control group were given blank matrix, and other drug groups were given external administration for 1 d, 2 times. The thickness of left and right ears was measured by micrometer for 15 days, the thickness difference of left and right ears was calculated, the weight of left and right ear slices was measured by electronic balance, and the pathological changes of mouse ear tissue were observed by HE staining. The serum levels of TNF- 伪, IL-2, IL-4, IL-12 and IL-18 were determined by Elisa. Results: thalidomide cream could reduce the ear thickness, reduce the inflammation of ears, increase the level of IL-2IL-4 and decrease the level of TNF- 伪 IL-12. Conclusion: thalidomide cream has a better therapeutic effect on dermatitis eczema and its mechanism may be related to regulating the balance of Th1 and Th2. Thalidomide cream is expected to be a new drug for treating chronic dermatitis eczema.
【作者单位】：南方医科大学皮肤病医院广东省皮肤病医院;
【基金】：广东省医学科学技术研究基金项目(编号:B2015037)
【分类号】：R965

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