硒矿区土壤中真菌的分离培养及抗肿瘤活性筛选

发布时间：2018-03-05 22:08

本文选题：真菌　切入点：硒矿床区　出处：《北京大学学报(自然科学版)》2017年06期 　论文类型：期刊论文

【摘要】：利用单菌落挑选与划线培养技术,从硒矿区土壤样品中分离纯化真菌。通过摇床发酵培养,提取制备发酵样品。采用MTT法,用MDA-MB-231细胞、Hela细胞、SMMC-7721细胞测试发酵样品的抗肿瘤活性。从采自湖北恩施全球唯一独立硒矿床区的6个不同土样中分离真菌73株。其中,对人宫颈癌Hela细胞的IC_(50)小于90μg/m L的菌株32株,对人乳腺癌MDA-MB-231细胞的IC_(50)小于90μg/m L的菌株13株,对人肝癌SMMC-7721细胞的IC_(50)小于90μg/m L的菌株9株。这些活性菌株为筛选抗肿瘤天然产物提供了良好的药源菌株。
[Abstract]:The fungi were isolated and purified from the soil samples of selenium mining area by single colony selection and underlined culture. The fermentation samples were extracted and prepared by shaking table fermentation. MTT method was used to isolate and purify the fungi from the soil samples. The antitumor activity of fermentative samples was tested by MDA-MB-231 cell line Hela cell line SMMC-7721. 73 strains of fungi were isolated from 6 different soil samples collected from the only independent selenium deposit area in Enshi, Hubei Province. For human cervical cancer Hela cells, 32 strains were less than 90 渭 g / mL, and 13 strains for human breast cancer MDA-MB-231 cells were less than 90 渭 g / mL. Nine strains of SMMC-7721 cells with less than 90 渭 g / mL were isolated from human hepatoma SMMC-7721 cells. These active strains provided a good drug source for screening natural anti-tumor products.
【作者单位】：生物资源保护与利用湖北省重点实验室湖北民族学院武陵山天然药物研究所;国家环境保护饮用水水源地管理技术重点实验室深圳市环境科学研究院;
【基金】：湖北省高校优势特色学科群建设计划(鄂学位[2015]5) 生物资源保护与利用湖北省重点实验室重点项目(PKLHB1504) 深圳市科技研发资金(JCYJ20130402094730839) 湖北民族学院博士启动基金(4138004)和湖北民族学院大学生创新创业项目(2016CX0715)资助
【分类号】：R915

【相似文献】