肌醇、D-手性肌醇抗衰老机理的研究

发布时间：2018-03-07 17:48

本文选题：肌醇　切入点：D-手性肌醇　出处：《天津科技大学》2014年硕士论文　论文类型：学位论文

【摘要】：肌醇是维生素B族中的维生素,肌醇与胆碱一样是亲脂肪性的维生素,又称作环己六醇,肌醇是人类细胞生长和存活的必须营养素,其能生成磷酸肌醇酯,发挥类似胆碱的作用,降血脂并促进肝脏中脂肪的代谢。D-手性肌醇(D-chiro-inositol, DCI)是肌醇的旋光异构体,D-手性肌醇对大幅度促进胰岛素产生、降低血脂、对于PCOS患者提高排卵频率都有很好的疗效。它也具有抗氧化、抗老化和消炎的的功效。DCI的生产主要是从化学制剂或天然产物提取,既费时又昂贵。本课题首先对肌醇和D-手性肌醇进行纯度检测,然后采用D-半乳糖致衰老的模型小鼠,来研究肌醇和DCI的抗衰老功能。比较其空间结构的不同在体内抗氧化活性的差异。ICR小鼠经颈背部皮下注射D-半乳糖后建立衰老小鼠模型,分别以100、200、400/(kg·bw)剂量的肌醇和DCI灌胃。干预八周后,对小鼠进行行为学实验,包括紧绳实验、平衡木实验、旷场实验和水迷宫实验,结果表明肌醇和DCI的中、高组剂量小鼠对陌生环境中的自发运动能力、探索运动能力、兴奋性和运动性性、空间记忆都显著好于模型对照组。小鼠处死后,检测并比较血清、肝脏和脑组织中过氧化氢酶(CAT)的活性、超氧化物歧化酶(SOD)的活性、总抗氧化能力(T-AOC)、谷胱甘肽过氧化物酶(GSH-Px)的活性及丙二醛(MDA)的含量并通过Real Time-PCR法检测肝脏中抗氧化相关基因mRNA表达水平。酶活测定结果显示：与模型组小鼠相比,不同剂量的肌醇和D-手性肌醇能不同程度增加小鼠血清和组织中的SOD、CAT和GSH-Px的活性,提高T-AOC,降低MDA含量。说明肌醇和DCI可以通过提高抗氧化酶的活性,显著提高D-半乳糖致衰老小鼠的抗氧化能力,其中肌醇和DCI在较高剂量干预时效果最好。Real Time-PCR结果显示：肌醇和DCI中、高剂量组小鼠和模型对照组相比,肝脏组织中SOD1、SOD2、GSH-Px和CAT基因的相对表达量均表现出显著性升高。
[Abstract]:Inositol is a vitamin in the B group of vitamins. Inositol, like choline, is a lipophilic vitamin, also known as cyclohexanol. Inositol is a necessary nutrient for the growth and survival of human cells, and it produces creatine phosphate. It plays a choline-like role in lowering blood lipids and promoting lipid metabolism in the liver. D-chiro-inositol (DCI), the isomer of inositol, greatly promotes insulin production and reduces blood lipids. It also has antioxidant, anti-aging and anti-inflammatory effects. DCI is produced mainly from chemicals or natural products. In this study, the purity of inositol and D-chiral inositol was detected firstly, and then the aging model mice induced by D-galactose were used. To study the anti-aging function of inositol and DCI. To compare the difference of antioxidation activity between inositol and DCI in vivo. ICR-mice were injected subcutaneously with D-galactose into the neck and back to establish aging mice model. Inositol and DCI were administered at the dose of 100g / 200g / kg 路BW, respectively. After eight weeks of intervention, behavioral experiments were carried out in mice, including tight-rope test, balance beam test, open field test and water maze test. The results showed that inositol and DCI were moderate. The spontaneous motor ability, exploring motor ability, excitability, motor ability and spatial memory of mice in high dose group were significantly better than those in model control group. After the mice were killed, the serum was detected and compared. The activity of catalase (catalase) and superoxide dismutase (SOD) in liver and brain. The activity of T-AOCX, glutathione peroxidase (GSH-Px) and the content of malondialdehyde (MDA) in liver were determined by Real Time-PCR assay. Different doses of inositol and D-chiral inositol could increase the activities of cat and GSH-Px in serum and tissues of mice, increase T-AOC and decrease the content of MDA. It was suggested that inositol and DCI could increase the activity of antioxidant enzymes. The anti-oxidation ability of aging mice induced by D-galactose was significantly increased. The results showed that inositol and DCI had the best effect on aging mice at higher dose. Real Time-PCR results showed that in the high dose group and DCI group, the mice in high dose group were compared with the model control group. The relative expression of GSH-Px and CAT in liver tissues was significantly increased.
【学位授予单位】：天津科技大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R965

【相似文献】