新型负载多柔比星纳米基因载体的研制及其对骨肉瘤细胞的抑制效应

发布时间：2018-03-08 22:33

本文选题：葡聚糖　切入点：聚乙烯亚胺　出处：《中国新药杂志》2016年09期 　论文类型：期刊论文

【摘要】：目的:研究制备葡聚糖(DEX)-多柔比星(ADM)-聚乙烯亚胺(PEI)纳米材料,同时将livinshRNA质粒和ADM转入骨肉瘤细胞,观察其对骨肉瘤的抑制效应。方法:制备了纳米基因载体并进行了表征,用紫外光谱、核磁共振和红外光谱分析测定了这些纳米颗粒的组合物,倒置荧光显微镜和流式细胞术证实了转染效率。应用MTT法检测了ADM和livin基因介导的协同作用,及其纳米颗粒对骨肉瘤细胞的抑制作用。结果:紫外光谱和核磁共振显示纳米载体出现ADM的特征峰,ADM的载药率为(17.43±0.548)%,该载体能有效地将livin shRNA质粒转入骨肉瘤细胞中。转染后骨肉瘤细胞存活率显著低于游离ADM组和纳米基因载体组(P0.05)。纳米基因载体组对骨肉瘤细胞的增殖抑制作用显著高于ADM组(P0.05)。结论:本研究中DEX-ADM-PEI纳米能有效地将ADM和livin shRNA质粒同时转入骨肉瘤细胞中,对骨肉瘤细胞增殖的协同抑制作用显著高于ADM。
[Abstract]:Objective: to study the preparation of dextran (DEX) -doxorubicin (DEX) -doxorubicin (DEX) -doxorubicin (DEX) -doxorubicin (DEX) -doxorubicin (DEX) -doxorubicin (DEX) -doxorubicin (DEX) -doxorubicin (DEX) -doxorubicin (D@@. The composition of these nanoparticles was determined by UV, NMR and IR spectra. The transfection efficiency was confirmed by inverted fluorescence microscope and flow cytometry. The synergistic effect of ADM and livin gene was detected by MTT method. Results: UV and NMR showed that the drug loading rate of ADM was 17.43 卤0.548%. The vector could effectively transfer livin shRNA plasmid into osteosarcoma cells. After transfection, the survival rate of osteosarcoma cells was significantly lower than that of free ADM group and nano gene carrier group (P 0.05). The inhibitory effect of nano gene carrier group on osteosarcoma cell proliferation was significantly higher than that of ADM group. Conclusion: in this study, DEX-ADM-PEI nanoparticles can effectively inhibit the proliferation of osteosarcoma cells. The plasmids of ADM and livin shRNA were transferred into osteosarcoma cells at the same time. The synergistic inhibitory effect on osteosarcoma cell proliferation was significantly higher than that on ADM.
【作者单位】：南昌大学第二附属医院骨科;复旦大学附属中山医院骨科;华东理工大学材料与工程学院;
【基金】：江西省科技厅科技社会发展项目(20122BBG70107-3) 江西省卫生计生委科技计划(20155278)
【分类号】：R943

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