海洋放线菌Y12-26中抗真菌活性代谢产物的分离纯化与结构鉴定

发布时间：2018-03-09 15:22

本文选题：海洋放线菌　切入点：分离纯化　出处：《中国抗生素杂志》2017年08期 　论文类型：期刊论文

【摘要】：目的研究海洋放线菌Y12-26发酵液中对几种植物病原真菌具有抑制作用的次生代谢活性物质。方法发酵离心上清液用等体积正丁醇萃取,得到发酵液浸膏。浸膏采用正相硅胶柱色谱、制备TLC和半制备HPLC等方法进行分离纯化该菌株活性次级代谢产物,并运用MS、~1H NMR和~(13)C NMR等波谱技术并结合文献比对鉴定所分离的单体化合物。结果从28g正丁醇萃取物中分离纯化并鉴定了3个单体活性化合物,分别为:salicylamide(1)、iturin A-2(2)、daidzein-4',7-di-α-L-rhamnoside(3)。结论海洋放线菌Y12-26能代谢产生具有多种生物活性的化合物。化合物1对Botriytis cinerea和Pyricularia oryzae的最低抑菌浓度(minimal inhibitory concentration,MIC)为125μg/mL,化合物2有强烈的抗真菌活性,其对Rhizoctonia solani的MIC为12.5μg/mL,化合物3对Botriytis cinerea的MIC为125μg/mL。本文首次报道大豆素类化合物3抗植物病原真菌活性。
[Abstract]:Objective to study the secondary metabolite active substances in the fermentation broth of marine actinomycetes Y12-26, which can inhibit several plant pathogenic fungi. Methods the centrifuge supernatant was extracted with n-butanol of equal volume. The yeast extract was obtained. The secondary metabolites of the strain were separated and purified by normal phase silica gel column chromatography, preparation of TLC and semi-preparation of HPLC. The isolated monomers were identified by means of MS 1H NMR and 13C NMR. Results three monomer active compounds were isolated and identified from 28g n-butanol extract. Conclusion Marine actinomycetes Y12-26 can metabolize compounds with various biological activities. The minimum inhibitory concentration of compound 1 to Botriytis cinerea and Pyricularia oryzae is 125 渭 g / mL, and compound 2 has strong antifungal activity. Its MIC to Rhizoctonia solani was 12.5 渭 g / mL, and the MIC of compound 3 to Botriytis cinerea was 125 渭 g / mL. The activity of daidzein compound 3 against phytopathogenic fungi was reported for the first time.
【作者单位】：华东理工大学上海市化学生物学(芳香杂环)重点实验室;
【基金】：国家科技支撑项目(No.2011BAE06B04-1)
【分类号】：R915

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