高效液相色谱法同时测定人尿液中布洛芬和吲哚美辛的浓度

发布时间：2018-03-10 13:40

本文选题：高效液相色谱法　切入点：布洛芬　出处：《中国药房》2017年26期 　论文类型：期刊论文

【摘要】：目的:建立同时测定人尿液中布洛芬和吲哚美辛浓度的方法。方法:尿液样品经乙腈沉淀蛋白后,采用高效液相色谱法测定。色谱柱为Discovery C_(18),流动相为乙腈-20 mmol/L乙酸铵溶液(85∶15,V/V,用冰醋酸调节pH至3.5),紫外检测波长为220nm,流速为1.0 mL/min,柱温为室温,进样量为80μL。结果:布洛芬和吲哚美辛的尿药浓度均在0.1~50.0μg/mL范围内线性关系良好(r分别为0.999 6、0.999 5,n=3),定量下限均为0.1μg/mL,最低检测限均为0.03μg/mL;日内、日间RSD均小于10%(n=5),准确度为94.7%~97.2%;布洛芬和吲哚美辛的提取回收率分别为89.5%~91.8%、90.2%~92.4%(RSD均小于10%,n=15)。结论:该方法简便、快速,且选择性、精密度、准确度良好,适用于人尿液中布洛芬和吲哚美辛浓度的同时测定。
[Abstract]:Objective: to establish a method for simultaneous determination of ibuprofen and indomethacin in human urine. High performance liquid chromatography (HPLC) was used. The chromatographic column was Discovery Che / L 18, the mobile phase was acetonitrile-20 mmol/L ammonium acetate solution 85: 15 V / V, pH was adjusted to 3.5 渭 m with glacial acetic acid, UV detection wavelength was 220 nm, flow rate was 1.0 mL / min, column temperature was room temperature, Results: the concentration of ibuprofen and indomethacin in urine were linear in the range of 0.1 渭 g / mL, r = 0.999 6, 0.999 5 渭 g / mL, respectively, and the lower limit of quantification was 0.1 渭 g / mL, the lowest detection limit was 0.03 渭 g / mL, and the limit of detection was 0.03 渭 g / mL, respectively, in the range of 0.1 渭 g / mL for ibuprofen and indomethacin. The recoveries of ibuprofen and indomethacin were 89.5% and 91.2% respectively. Conclusion: this method is simple, rapid, selective, precise and accurate. It is suitable for simultaneous determination of ibuprofen and indomethacin in human urine.
【作者单位】：咸宁市中医医院药房;重庆医科大学药学院;重庆医科大学检验医学院/临床检验诊断学教育部重点实验室;
【基金】：国家自然科学基金资助项目(No.21672031)
【分类号】：R969

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