小鼠背海马synaptotagminⅠ和GFAP蛋白含量的年龄效应及长期口服阿卡波糖的影响

发布时间：2018-03-10 22:41

  本文选题：小鼠　切入点：衰老　出处：《安徽医科大学》2014年硕士论文　论文类型：学位论文

【摘要】：背景 随着国内人口老龄化的加速，如何延缓衰老及衰老相关的认知功能下降从而改善老年人生活质量已成为研究的热点。Synaptotagmin (Syt) Ⅰ属于突触前囊泡蛋白，参与调控突触囊泡同步的快速胞吐过程。当前的研究显示老年快速老化小鼠（SAMP8）背侧海马SytⅠ蛋白升高，并且和年龄相关的空间学习和记忆障碍相关。胶质原纤维酸性蛋白(Glial Fibrillary Acidic Protein，GFAP)是星形胶质细胞中间丝的主要蛋白，是其特异性生物标志物，它的表达随着年龄的增长而升高。大量的研究表明，星形胶质细胞和学习、记忆及认知功能，特别是各种年龄相关的神经变性疾病，，如阿尔茨海默氏病、帕金森病密切相关。阿卡波糖作为α-糖苷酶抑制剂，长期口服具有稳定餐后及空腹血糖、抑制晚期糖基化终末产物生成的作用。它也有预防和延缓衰老相关疾病如2型糖尿病及心血管疾病的发生发展作用。这些提示阿卡波糖可能具有延缓衰老效应。 目的 探讨长期口服阿卡波糖对老年SAMP8小鼠背海马SytⅠ和GFAP蛋白含量的影响。 方法 实验动物先随机分成老年空白对照组和阿卡波糖组。所有小鼠自由饮食。阿卡波糖组小鼠从3月龄开始随水给予阿卡波糖，至9月龄时再加入一批3月龄青年组小鼠作为青年组，与先前两组小鼠同时进行行为学任务检测，选用免疫组化技术检测3组SAMP8小鼠背海马各区中Syt Ⅰ和GFAP表达。 结果 ①所有受试小鼠背海马齿状回、CA1区及CA3区内Syt Ⅰ和GFAP蛋白均有表达；②与青年对照组相比，老年对照组小鼠齿状回颗粒细胞层和分子层、CA1区及CA3区各层SytⅠ相对含量均显著升高（Ps＜0.05）；阿卡波糖处理组小鼠齿状回颗粒细胞层、分子层以及CA1和CA3区各层的SytⅠ相对含量均显著低于同龄对照组（Ps＜0.05）；③与青年对照组相比，老年对照组小鼠齿状回、CA1区及CA3区GFAP相对含量均显著升高（Ps＜0.05）；阿卡波糖处理组小鼠CA1区和 CA3区的GFAP相对含量均显著低于同龄对照组（Ps＜0.05）。 结论①在SAMP8小鼠背海马各区亚层内，SytⅠ和GFAP含量呈不同程度的年龄相关性增加；②长期口服阿卡波糖可以减轻SAMP8小鼠其背海马各亚层内SytⅠ和GFAP的衰老效应。
[Abstract]:Background. With the acceleration of the aging population in China, how to delay senescence and the decline of cognitive function related to aging and improve the quality of life of the elderly has become a hot topic. Synaptotagmin SytI belongs to the presynaptic vesicle protein. It is involved in the regulation of rapid exocytosis in synaptic vesicle synchronization. Recent studies have shown that the Syt I protein in the dorsal hippocampus of aged mice with rapid aging is increased. The glial fibrillary acidic protein (Glial Fibrillary Acidic protein GFAP) is the main protein of astrocytic mesenchyme and a specific biomarker. A large number of studies have shown that astrocytes and learning, memory and cognitive functions, especially age-related neurodegenerative diseases, such as Alzheimer's disease, increase with age. Parkinson's disease is closely related. As an inhibitor of 伪 -glucosidase, acarbose has stable postprandial and fasting blood glucose for a long time. It can also prevent and delay the occurrence and development of aging related diseases such as type 2 diabetes and cardiovascular diseases. These results suggest that acarbose may have the effect of delaying senescence. Purpose. To investigate the effects of long-term oral acarbose on the contents of Syt 鈪

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