瑞舒伐他汀对内皮细胞功能变化的影响

发布时间：2018-03-11 07:37

本文选题：瑞舒伐他汀　切入点：内皮细胞　出处：《中国药房》2015年29期 　论文类型：期刊论文

【摘要】：目的:研究瑞舒伐他汀对内皮细胞功能变化的影响。方法:选择2013年6月-2014年6月我院健康体检者15例,冠心病患者30例。分成健康对照组、冠心病药物干预组、冠心病组,各15例。对比各组不对称二甲基精氨酸(ADMA)、一氧化氮(NO)及一氧化氮合酶(NOS)的血液酶联免疫吸附测定(ELISA)检测结果,分析酶标仪光密度(OD)值与其相关性;对人脐静脉内皮细胞(HUVEC)培养4 h及8 h后,根据瑞舒伐他汀的浓度剂量分成5个组,即0μmol/L对照组和0.1、1、10、100μmol/L组,检测不同浓度瑞舒伐他汀对内皮细胞二甲基精氢酸二甲胺水解酶(DDAH)信使核糖核酸(m RNA)的表达变化。结果:冠心病药物干预组的ADMA水平均显著低于冠心病组,但仍高于健康对照组;NO及NOS水平均显著高于冠心病组,但仍低于健康对照组,差异均有统计学意义(P0.05)。根据Pearson法分析相关性可知,酶标仪OD值与ADMA、NO及NOS均呈正相关。HUVEC细胞培养4 h后的逆转录-聚合酶链反应(RT-PCR)结果显示,0.1、1、10及100μmol/L瑞舒伐他汀组的DDAH m RNA表达均上升,且10μmol/L及100μmol/L瑞舒伐他汀组的DDAH m RNA表达较对照组的差异有统计学意义(P0.05)。培养8 h后的RT-PCR结果显示,0.1、1、10及100μmol/L瑞舒伐他汀组的DDAH m RNA表达均上升,且10μmol/L瑞舒伐他汀组的DDAH m RNA表达较对照组的差异有统计学意义(P0.05)。结论:瑞舒伐他汀对内皮细胞具有较好的保护作用,值得临床关注与重视。
[Abstract]:Objective: to study the effect of Risuvastatin on endothelial cell function. Methods: 15 healthy persons and 30 patients with coronary heart disease were selected from June 2013 to June 2014 in our hospital. They were divided into healthy control group, coronary heart disease intervention group and coronary heart disease group. The results of blood enzyme linked immunosorbent assay (ELISAs) of asymmetric dimethyl arginine (ADMAN) and nitric oxide synthase (NOS) were compared in each group. Human umbilical vein endothelial cells (HUVECs) were cultured for 4 h and 8 h. According to the concentration of resuvastatin, they were divided into 5 groups: 0 渭 mol/L control group and 0.1 渭 mol/L 10 渭 mol/L group. To detect the expression of Risuvastatin in endothelial cells, the level of ADMA in the drug intervention group was significantly lower than that in the coronary heart disease group. But the levels of no and NOS were significantly higher than those of the healthy control group, but still lower than that of the healthy control group. The difference was statistically significant (P 0.05). The OD value of enzymatic marker was positively correlated with ADMA-no and NOS. The results of reverse transcription-polymerase chain reaction (RT-PCRR) showed that the expression of DDAH m RNA was increased in the group of 0.1 渭 mol/L and 100 渭 mol/L resuvastatin. The expression of DDAH m RNA in the 10 渭 mol/L and 100 渭 mol/L resuvastatin groups was significantly higher than that in the control group (P 0.05). The results of RT-PCR culture for 8 h showed that the DDAH m RNA expression in the 10 渭 mol/L and 100 渭 mol/L resuvastatin groups was increased. The expression of DDAH m RNA in the 10 渭 mol/L resuvastatin group was significantly higher than that in the control group (P 0.05). Conclusion: rosuvastatin has a better protective effect on endothelial cells and deserves clinical attention.
【作者单位】：复旦大学附属金山医院心血管内科;
【基金】：上海市金山区科学技术委员会科学技术创新基金项目(No.2012-3-04)
【分类号】：R96

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