罗红霉素对小鼠乙酰胆碱预收缩气管环的舒张作用

发布时间：2018-03-11 12:31

本文选题：气管环　切入点：乙酰胆碱　出处：《山东医药》2017年26期 　论文类型：期刊论文

【摘要】：目的观察罗红霉素对小鼠气管环的舒张作用,并探讨其作用机制。方法取BALB/c小鼠15只,处死后分离气管环,将气管环置于灌流槽中,经过高钙液平衡后开始实验。使用激动剂乙酰胆碱(Ach)100μmol/L收缩气管环,待气管环张力达到平台期后,分别加入终浓度为10~(-2)、10~(-1.5)、10~(-1)、10~(-0.5)、10~0、10~(0.5)、10~(0.75)、10~1mg/mL的罗红霉素,观察不同浓度罗红霉素作用下的气管环肌张力和舒张比率,将引起气管环最大舒张的最低药物浓度10~(0.75)mg/mL作为罗红霉素最佳药物浓度。向灌流槽中加入100μmol/L Ach预收缩气管环,随后加入L型钙离子通道抑制剂硝苯地平、非选择性阳离子通道抑制剂吡唑盐3(Pyr3),待抑制剂舒张气管作用达到平台期后,加入10~(0.75)mg/mL罗红霉素,观察气管环的肌张力变化和舒张比率。结果 Ach预收缩的气管环的肌张力为(7.03±0.35)mN,加入最佳浓度的罗红霉素后,肌张力降至(3.47±0.29)mN(P0.05)。加入硝苯地平、Pyr3后气管环的肌张力降低,舒张比率增加(P均0.05);继续加入罗红霉素后,气管环的肌张力继续降低,舒张比率较前升高(P均0.05)。结论罗红霉素对小鼠Ach预收缩的气管环具有舒张作用,并可增强硝苯地平、Pyr3对于Ach预收缩气管环的舒张作用,其主要通过调控钙离子通道来发挥作用。
[Abstract]:Objective to observe the relaxation effect of roxithromycin on tracheal rings in mice and to explore its mechanism. After the high calcium solution balance, the experiment began. The agonist acetylcholine acetylcholine acetylcholine #number0# 渭 mol/L was used to contract the trachea ring. After the tension of the trachea ring reached the plateau stage, the final concentration of roxithromycin was 10 ~ (10) ~ 10 ~ (-1) ~ (10 ~ (10)) ~ (10 ~ (10)) ~ (10 ~ (10)) (n = 10). To observe the tension and relaxation ratio of tracheal ring muscle under different concentrations of roxithromycin, the lowest drug concentration (10 ~ 0.75 mg / mL) was used as the best concentration of roxithromycin, and the 100 渭 mol/L Ach precontracted tracheal ring was added to the perfusion tank. The L-type calcium channel inhibitor nifedipine and the nonselective cationic channel inhibitor pyrazolium 3 were added. After the relaxation of trachea reached the plateau stage, the inhibitor was added with roxithromycin 0.75 mg / mL. Results the muscle tension of tracheal rings precontracted by Ach was 7.03 卤0.35 mN.The muscle tension of tracheal rings decreased to 3.47 卤0.29 mNP0.05 after adding roxithromycin at the optimum concentration, and the muscle tension of tracheal rings decreased after adding nifedipine to Pyr3. After adding roxithromycin, the muscle tension of tracheal rings continued to decrease and the relaxation ratio increased to 0.05%. Conclusion roxithromycin has a relaxing effect on Ach precontracted tracheal rings in mice. The relaxation effect of nifedipine PYR3 on Ach precontracted tracheal rings was enhanced by regulating calcium channels.
【作者单位】：中南民族大学生命科学学院;
【基金】：国家自然科学基金面上项目(31571200)
【分类号】：R965

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