RP-HPLC法测定紫杉醇脂质体的药物包封率

发布时间：2018-03-13 00:27

本文选题：红豆杉　切入点：二萜生物碱类化合物　出处：《药物分析杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的:建立紫杉醇脂质体药物包封率的测定方法。方法:采用葡聚糖凝胶G-50柱色谱法分离紫杉醇脂质体和游离药物,用RP-HPLC法检测紫杉醇的量,计算脂质体中紫杉醇的包封率;色谱条件:采用Platisil-ODS(250 mm×4.6 mm,5μm)色谱柱,以甲醇-水(含0.1%三乙胺和0.67%冰乙酸)(80∶20)为流动相,流速1.0mL·min~(-1),检测波长227 nm,柱温为室温。结果:紫杉醇与辅料及溶剂的色谱峰分离良好,峰形很好;紫杉醇线性范围0.5~50μg·mL~(-1)(r=0.999 3),加样回收率范围为99.66%~100.8%,包封率范围为90.29%~91.65%。结论:本方法能快速检测紫杉醇脂质体中紫杉醇含量及包封率。
[Abstract]:Objective: to establish a method for the determination of drug entrapment efficiency of paclitaxel liposomes. Methods: paclitaxel liposomes and free drugs were separated by dextran gel G-50 column chromatography, the amount of paclitaxel was detected by RP-HPLC, and the entrapment efficiency of paclitaxel in liposomes was calculated. The chromatographic conditions were as follows: Platisil-ODS(250 mm 脳 4.6mm ~ 5 渭 m) column, methanol-water (containing 0.1% triethylamine and 0.67% glacial acetic acid 80: 20) as mobile phase, flow rate 1.0 mL 路min ~ (-1), detection wavelength 227nm, column temperature as room temperature. Results: the chromatographic peak of taxol, excipients and solvents was well separated and the peak form was very good. The linear range of paclitaxel was 0.5 渭 g 路mL~(-1)(r=0.999 _ 3, the recovery range was 100.8 and the entrapment efficiency was 90.29.5 渭 g 路mL~(-1)(r=0.999 _ 3.Conclusion: this method can be used for rapid determination of paclitaxel content and encapsulation efficiency in paclitaxel liposomes.
【作者单位】：广东药科大学中药学院;广州市茗川生物科技有限公司;
【基金】：国家自然科学基金(81303288) 国家科技创新基金(国科发计[2013]583号) 广东省教育厅创新强校特色创新项目(粤教科函[2015]3号)
【分类号】：R927;O657.72

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