自组装方法制备丝素微球及其结构与性能表征

发布时间：2018-03-13 19:04

本文选题：丝素微球　切入点：自组装　出处：《蚕业科学》2015年04期 　论文类型：期刊论文

【摘要】：为了简化丝素微球的制备方法及保证丝素微球作为药物缓释载体的安全性与结构稳定性,以丝素蛋白为原材料,采用自组装方法并调节丝素蛋白溶液浓度、冷冻温度、异丙醇添加量等条件,制备粒径在0.4~1.3μm之间的丝素微球。扫描电子显微镜(SEM)下观察制备的丝素微球呈规则的球状结构,球面光滑,粒径分布较为均匀;采用红外光谱仪(FT-IR)和热重分析仪(DTG)测试自组装后丝素微球的分子构象已发生转化,β-折叠结构含量明显增加,并具有较好的热稳定性。研究结果表明,丝素蛋白溶液浓度、冷冻温度、异丙醇添加量对丝素微球的形态结构有影响,可以通过自组装方法制备出粒径可控,结构和性能稳定的丝素微球,并有望开发为药物缓释载体。
[Abstract]:In order to simplify the preparation method of silk fibroin microspheres and ensure the safety and structural stability of silk fibroin microspheres as drug sustained-release carriers, silk fibroin was used as raw material, and the concentration of silk fibroin solution and freezing temperature were adjusted by self-assembly method. When isopropanol was added, fibroin microspheres with particle size of 0.4 渭 m or 1.3 渭 m were prepared. The prepared fibroin microspheres were characterized by regular spherical structure, smooth spherical surface and uniform particle size distribution under scanning electron microscopy (SEM). The molecular conformation of silk fibroin microspheres after self-assembly was measured by FT-IR and DTG. The 尾 -fold structure content was obviously increased, and the content of 尾 -fold structure was increased obviously. The results showed that the concentration of silk fibroin solution was higher than that of the control. Freezing temperature and the amount of isopropanol can affect the morphology and structure of fibroin microspheres. The fibroin microspheres with controllable particle size, stable structure and properties can be prepared by self-assembly method, and can be used as drug delivery carrier.
【作者单位】：浙江大学应用生物资源研究所;
【基金】：现代农业产业技术体系建设专项(No.CARS-22)
【分类号】：R943

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