阿奇霉素对大鼠胃排空及血浆胃动素影响的研究

发布时间：2018-03-15 03:07

本文选题：阿奇霉素　切入点：胃排空　出处：《兰州大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的：观察大鼠采用不同剂量阿奇霉素灌胃后,全胃半排空时间及血浆胃动素浓度的变化,揭示阿奇霉素致胃肠道症状的可能机制。方法： 80只雄性wistar大鼠随机分为对照组(8只)和观察组(72只)；观察组按给药剂量分为小剂量、中等剂量、大剂量组,每组按给药时间分为1d组、3d组、6d组。观察组大鼠采用阿奇霉素灌胃,小剂量组、中等剂量组、大剂量组各组灌药剂量分别为3mg/100g、6mg/100g、12mg/100g。所有大鼠实验当天先用SPECT行胃排空检测,试餐为含有99mTc-DTPA的牛奶,用专用软件分析原始采集数据,得到全胃半排时间(GET1/2)、50分钟末残留率百分比、胃排空曲线。胃排空检测结束后,经腹主动脉采集动脉血2m1,运用放免法测定血浆胃动素浓度。用统计软件SPSS17.0进行统计分析,计数资料以“均数±标准差”表示,p0.05为差异有显著性,p0.01为差异有极显著性意义。结果：小剂量组、中等剂量组、大剂量组全胃半排时间明显缩短,与正常对照组(64.21±7.24)min比较,有统计学意义(p0.05)。组内不同时间点比较无统计学意义(p0.05)。观察组血浆胃动素浓度,小剂量组与正常对照组比较无统计学意义(p0.05)；中剂量组、大剂量组与正常对照组比较明显增高,差异有统计学意义(p0.05)。结论：阿奇霉素可促进胃排空。阿奇霉素促进胃排空的机制可能为小剂量下仅为胃动素样作用；中等剂量、大剂量时除胃动素样作用外,有内源性胃动素参与。
[Abstract]:Objective:. The changes of total gastric emptying time and plasma motilin concentration after different doses of azithromycin were observed in rats to reveal the possible mechanism of gastrointestinal symptoms induced by azithromycin. Methods:. 80 male wistar rats were randomly divided into control group (n = 8) and observation group (n = 72). Each group was divided into 1 day group, 3 d group and 6 d group according to the time of administration. Rats in the observation group were given azithromycin intragastric perfusion, small dose group, middle dose group, high dose group and large dose group, the dosage of each group was 3 mg / 100 mg / 100 mg / 100 mg / 12 mg / 100 g respectively. All rats were tested for gastric emptying with SPECT on the day of the experiment. The test meal consisted of milk containing 99mTc-DTPA. By analyzing the original data with special software, the percentage of residual rate at the end of 50 minutes and the gastric emptying curve were obtained. After the detection of gastric emptying, the percentage of residual rate at the end of 50 minutes was obtained. The plasma motilin concentration was measured by radioimmunoassay (RIA). The count data were statistically analyzed by SPSS17.0. There was significant difference in the count data with "mean 卤standard deviation" (P 0.05). Results:. Compared with the control group (64.21 卤7.24 min), there was statistical significance (P 0.05). There was no significant difference between the two groups at different time points (P 0.05). The plasma motilin concentration in the observation group was significantly shorter than that in the control group (P < 0.05). There was no significant difference between the low dose group and the normal control group (P 0.05), while in the middle dose group, the large dose group was significantly higher than that in the normal control group, and the difference was statistically significant (P 0.05). Conclusion:. Azithromycin can promote gastric emptying. The mechanism of azithromycin promoting gastric emptying may be the action of motilin at low dose and endogenous motilin at middle dose and high dose.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R965

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