LC-MS同时测定大鼠脑脊液中谷氨酸和γ-氨基丁酸的方法研究

发布时间：2018-03-15 04:15

本文选题：液质联用法　切入点：谷氨酸　出处：《中国测试》2015年02期 　论文类型：期刊论文

【摘要】：建立LC-MS同时检测大鼠脑脊液中谷氨酸和γ-氨基丁酸的方法。采用安捷伦ZORBAX SIL(4.6 mm×250 mm×5μm)硅胶正相色谱柱,流动相为甲醇-水(0.1%甲酸)=50∶50(v/v),梯度洗脱,流速为1 m L/min。电喷雾离子源(eletrospray ionization,ESI)采用正离子化方式,以多重反应检测(multiple reaction monitoring,MRM)方式进行检测,用于定量分析的离子反应分别为m/z 148.1→m/z 84.1(谷氨酸,[M+H]+),m/z 104.1→m/z 87.1(γ-氨基丁酸,[M+H]+)。测定大鼠脑脊液中谷氨酸在500~2 500μg/L和γ-氨基丁酸在50~250μg/L质量浓度范围内,浓度与峰面积呈良好的线性关系。该方法简便、准确、灵敏度高,专属性强,重复性好,适用于脑脊液中谷氨酸和γ-氨基丁酸的同时测定,可为氨基酸类神经递质提供有效的检测手段。
[Abstract]:A LC-MS method for simultaneous determination of glutamic acid and 纬 -aminobutyric acid in cerebrospinal fluid of rats was established by using ZORBAX SIL(4.6 mm 脳 250mm 脳 5 渭 m silica gel positive phase chromatographic column. The mobile phase consisted of methanol-water 0.1% formic acid 50: 50 v / v 路v / v, gradient elution. The flow rate was 1 mL 路min. The electrospray ion source eletrospray ionizationn (ESI) was detected by positive ionization and multiple reaction monitoring by multiple reaction. The ion reaction used for quantitative analysis was m / z 148.1 respectively. 鈫扢 / z 84.1. 鈫扢 / z 87.1 (纬 -aminobutyric acid, [M H]). The linear relationship between the concentration of glutamic acid and peak area in the range of 500 ~ 2 500 渭 g / L of 纬 -aminobutyric acid and 50 ~ 250 渭 g / L of 纬 -aminobutyric acid in cerebrospinal fluid of rats is simple, accurate, sensitive and specific. It is suitable for simultaneous determination of glutamic acid and 纬 -aminobutyric acid in cerebrospinal fluid and can provide an effective method for the determination of amino acid neurotransmitters.
【作者单位】：成都医学院药学院;
【基金】：成都医学院药学重点学科建设项目(CYXK2012006)
【分类号】：O657.63;R927.2

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