高效液相色谱测定比格犬血浆中的头孢唑啉与头孢曲松的浓度

发布时间：2018-03-16 17:50

本文选题：高效液相色谱法　切入点：头孢唑啉　出处：《中国临床药理学杂志》2015年14期 　论文类型：期刊论文

【摘要】：目的建立HPLC在多项测定参数相同的基础上,分别测定比格犬血浆中头孢唑啉、头孢曲松浓度的方法。方法色谱柱:头孢唑啉为ES industries Epic C18(4.6 mm×150 mm,5μm),头孢曲松为Phenomenex Gemini C18(4.6 mm×150mm,5μm),流动相:头孢唑啉为甲醇-0.05 mol·L-1乙酸铵缓冲液(p H=4.3)=30∶70;头孢曲松为乙腈-0.05 mol·L-1KH2PO4缓冲液(p H=6.0)=6∶94,流速均为1.0 m L·min-1,进样量均为40μL。结果头孢唑啉标准曲线回归方程为y=-1.25×104x+8479.37(r=0.997 4),头孢曲松为y=1.25×104x+2.72×104(r=0.997 6)。头孢唑啉线性范围为2~300μg·m L-1,头孢曲松为2~200μg·m L-1;日内、日间精密度相对标准偏差均≤10%,提取回收率均≥85.78%。结论本法灵敏、准确、快速,可用于比格犬血浆头孢唑啉、头孢曲松浓度的测定和药物其他方面研究。
[Abstract]:Objective to establish a HPLC method for the determination of cefazolin in Beagle dog plasma on the basis of the same parameters. Method for determination of ceftriaxone concentration. Method: cefazolin was es industries Epic C184.6 mm 脳 150mm ~ 5 渭 m, ceftriaxone was Phenomenex Gemini C184.6 mm 脳 150mm ~ 5 渭 m, mobile phase was as follows: cefazolin was methanol -0.05 mol 路L ~ (-1) ammonium acetate buffer solution (p H4.3N) 3070; ceftriaxone was acetonitrile-0.05 mol 路L ~ (-1) KH _ 2PO _ 4 buffer solution p. Results the standard curve regression equation of cefazolin was you-1.25 脳 10 ~ 4x 8479.37rn ~ (0.997) ~ 4N, ceftriaxone = 1.25 脳 104x 2.72 脳 104rn ~ (0.997) ~ 6. The linear range of cefazolin and ceftriaxone were 2.300 渭 g 路ml ~ (-1) and 200 渭 g 路m ~ (-1), respectively, and the linear range of cefazolin and ceftriaxone were 2.300 渭 g 路ml ~ (-1) and 200 渭 g 路m ~ (-1), respectively, and the linear range of cefazolin was 2.25 脳 10 ~ (4) x 2.72 脳 10 ~ (4) ~ (-1) 渭 g 路m ~ (-1). Conclusion this method is sensitive, accurate and rapid, and can be used for the determination of cefazolin in plasma and ceftriaxone in beagle dogs.
【作者单位】：北京大学第一医院临床药理研究所;
【分类号】：R965

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