水杨酸盐毒性与大鼠耳蜗核炎症因子表达

发布时间：2018-03-19 02:08

本文选题：水杨酸盐　切入点：肿瘤坏死因子α(TNF-α)　出处：《中华耳科学杂志》2015年01期 　论文类型：期刊论文

【摘要】：目的本研究采用对大鼠急性及慢性水杨酸盐腹腔注射建立耳鸣动物模型,通过检测大鼠耳蜗核中肿瘤坏死因子α(TNF-α),白介素-1β(IL-1β),白介素6(IL-6)以及NMDA受体亚基(NR2B)m RNA及其蛋白的表达,了解慢性水杨酸盐诱导的耳鸣动物耳蜗核中是否出现炎症因子表达的改变,以探讨炎症因子在耳鸣中的作用。方法实验动物随机分为4组,按给药时间分别为:正常对照组、急性注射2小时组、慢性注射14天组、停药后恢复14天组。各组大鼠断头处死后迅速分离出耳蜗核;采用SYBR Green实时荧光定量PCR(real-time reverse transcriptase polymerase chain reaction,real-time RT-PCR)及Western blot(蛋白质印迹)方法,检测各组大鼠耳蜗核TNF-α,IL-1β,IL-6及NR2B基因m RNA及其蛋白的表达,观察各组间的差异。结果(1)在慢性注射14天组,TNF-α和NR2B m RNA及其蛋白表达水平较正常对照组、急性注射2小时组、停药后恢复14天组高,且差异有统计学意义(p0.05);急性注射2小时组、慢性注射14天组、停药后恢复14天组IL-1βm RNA水平较正常组高,慢性注射14天组、停药后恢复14天组IL-1β蛋白水平较正常组高,差异有统计学意义(p0.05);IL-6 m RNA及其蛋白表达水平在四组之间无明显差异;(2)TNF-α和NR2B m RNA表达具有明显的正相关(p0.01);IL-1β和NR2B m RNA表达无明显正相关(p0.05)。结论(1)长期注射水杨酸盐可能通过上调耳蜗核中TNF-α,IL-1β和NR2B基因表达导致大鼠耳鸣;(2)TNF-α和NR2B可能在水杨酸盐诱发耳鸣的过程中起协同作用。
[Abstract]:Objective to establish an animal model of tinnitus by intraperitoneal injection of acute and chronic salicylate in rats, and to detect the expression of tumor necrosis factor- 伪 (TNF- 伪), interleukin-1 尾 (IL-1 尾), interleukin-6 (IL-6) and NMDA receptor subunit NR2Bm RNA and their proteins in the cochlear nucleus of rats. To investigate the expression of inflammatory factors in the cochlear nucleus of tinnitus rats induced by chronic salicylate, and to explore the role of inflammatory factors in tinnitus. The cochlear nucleus was isolated quickly after decapitation, and the cochlear nucleus was rapidly isolated by SYBR Green real-time quantitative PCR(real-time reverse transcriptase polymerase chain reactionation RT PCR and Western blot (Western blotting). The expression of TNF- 伪 IL-1 尾 -guan6 and NR2B gene m RNA and their proteins in the cochlear nucleus of rats in each group were detected and the differences were observed. Results 1) the expression levels of TNF- 伪 and NR2B m RNA and their protein in the chronic injection group were higher than those in the normal control group and acute injection group for 2 hours. The levels of IL-1 尾 m RNA were significantly higher in the acute injection group (2 hours), chronic injection group (14 days group) than those in the control group (P < 0.05), and the level of IL-1 尾 m RNA in the 14 day group was higher than that in the control group. The level of IL-1 尾 protein in the group of 14 days after withdrawal was higher than that in the normal group. There was no significant difference in the expression of IL-6 m RNA and its protein between the four groups. There was a significant positive correlation between the expression of TNF- 伪 and NR2B m RNA. There was no significant positive correlation between the expression of IL-1 尾 and NR2B m RNA. Conclusion the expression of NR2B m RNA and IL 1 尾 are not positively correlated with the expression of TNF- 伪 and NR2B m RNA. Conclusion the expression of TNF- 伪 and NR2B m RNA can be treated with salicylate for a long time. The expression of TNF- 伪 IL-1 尾 and NR2B gene in the cochlear nucleus can induce TNF- 伪 and NR2B in rat tinnitus. TNF- 伪 and NR2B may play a synergistic role in the process of tinnitus induced by salicylate.
【作者单位】：上海交通大学医学院附属新华医院耳鼻咽喉-头颈外科;上海市耳鼻疾病转化医学重点实验室;
【基金】：国家自然科学基金项目(No.81371091,NO.81170917) 上海市教委重点项目(No.12ZZ103)
【分类号】：R965

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