6株红树林根际土壤放线菌的分离鉴定及活性测定

发布时间：2018-03-19 14:37

本文选题：红树林　切入点：放线菌　出处：《中国病原生物学杂志》2017年06期 　论文类型：期刊论文

【摘要】：目的分离和鉴定具有抗菌活性的红树林根际土壤放线菌,以期发现具有药用价值的放线菌资源。方法采用4种分离培养基以稀释涂布法分离放线菌,对分离自红树林根际土壤放线菌进行形态特征观察、生理、生化和和抗菌活性测定;采用PCR技术测定16SrRNA基因序列,进行比对分析和系统发育分析。结果从红树林根际土壤样品中分离出6株典型放线菌,其中BLHK5、BLHK125、BLHK198、BLHK245、BLHK271具有广谱抗菌活性,抑菌圈直径为926mm;根据其形态特征、生理生化和基于16SrRNA系统发育分析,初步鉴定BLHK125、BLHK271为小单孢菌属(同源性分别为99.83%和98.53%),BLHK217为糖单孢菌属(同源性为98.88%),BLHK5、BLHK198、BLHK245为链霉菌属(同源性为99.10%100.00%)。结论广西北部湾北仑河口红树林根际土壤放线菌资源丰富,从红树林根际土壤样品中分离的5株放线菌的发酵液浓缩物具有较好的抗菌活性,具有研究价值。
[Abstract]:Objective to isolate and identify actinomycetes from the rhizosphere soil of mangroves with antibacterial activity in order to find the resources of actinomycetes with medicinal value. The morphology, physiology, biochemistry and antibacterial activity of actinomycetes isolated from the rhizosphere of mangroves were observed, and 16s rRNA gene sequences were sequenced by PCR. Results six typical actinomycetes were isolated from the rhizosphere soil samples of mangrove forests. Among them, BLHK5 (BLHK5) BLHK125N BLHK198 BLHK245B BLHK271 have broad-spectrum antibacterial activity and the diameter of inhibition circle is 926mm. Physiological, biochemical and phylogenetic analysis based on 16s rRNA, BLHK125N BLHK271 was identified as Micromonosporium (homology 99.83% and 98.53, respectively). BLHK217 was glycomonas (the homology was 98.888k5HK5 HK198BLHK245) for Streptomyces. Conclusion there are abundant actinomycetes in the rhizosphere soil of the Beilun mangrove estuary in Beibu Bay, Guangxi, with the homology of 99.10. 000.Conclusion there are plenty of actinomycetes in the rhizosphere soil of the Beibu Bay, Guangxi. The fermentation broth concentrate of 5 strains of actinomycetes isolated from the soil samples of mangrove rhizosphere has good antibacterial activity and has research value.
【作者单位】：桂林医学院基础医学院;
【分类号】：R915

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