山柰酚对脂多糖诱导的肥大细胞炎症反应的抑制作用

发布时间：2018-03-19 20:19

本文选题：山柰酚　切入点：HMC-肥大细胞　出处：《药学学报》2015年06期 　论文类型：期刊论文

【摘要】：研究山柰酚对脂多糖(lipopolysaccharide,LPS)诱导的HMC-1肥大细胞炎症反应的抑制作用。利用MTT法检测山柰酚对HMC-1细胞的细胞毒性作用,同时确定山柰酚的给药浓度;ELISA检测活化的HMC-1细胞经不同浓度的山柰酚(10、20和40μmol·L-1)作用后组胺、IL-6、IL-8、IL-1β和TNF-α的释放量;Western blot检测经不同浓度的山柰酚作用于活化的HMC-1细胞后,胞质中p-IKKβ、IκBα、p-IκBα和胞核中NF-κB(p65)蛋白水平。根据MTT法的实验结果,确定了山柰酚的给药浓度范围为5~40μmol·L-1。山柰酚能够使活化的HMC-1肥大细胞中组胺、IL-6、IL-8、IL-1β和TNF-α的释放量显著下降(P0.01)。山柰酚作用于LPS诱导的HMC-1细胞后,p-IKKβ、p-IκBα和胞核中NF-κB(p65)蛋白水平会显著下降(P0.01)。结果表明,山柰酚对于肥大细胞炎症反应具有显著的抑制效应,它能够抑制IKKβ的活化,抑制IκBα的磷酸化,阻止NF-κB(p65)进入细胞核内,进而影响相关炎症介质的释放。
[Abstract]:To study the inhibitory effect of kaempferol on HMC-1 mast cell inflammation induced by lipopolysaccharide (LPS), the cytotoxicity of kaempferol to HMC-1 cells was detected by MTT assay. At the same time, the concentration of kaempferol was determined by Elisa to detect the release of IL-1 尾 and TNF- 伪 in activated HMC-1 cells treated with different concentrations of kaempferol 1020 and 40 渭 mol 路L ~ (-1). Western blot was used to detect the effect of kaempferol at different concentrations on activated HMC-1 cells. The levels of p-IKK 尾 -I 魏 B 伪 -p-I 魏 B 伪 and NF- 魏 B p65) protein in cytoplasm were determined by MTT assay. The concentration range of kaempferol was determined to be 540 渭 mol 路L -1. Kaempferol could significantly decrease the release of IL-1 尾 and TNF- 伪 in activated HMC-1 mast cells. Kaempferol could significantly decrease the levels of p-IKK 尾 p-I 魏 B 伪 and nuclear NF- 魏 Bp65 in HMC-1 cells induced by LPS. The results show that, Kaempferol has a significant inhibitory effect on mast cell inflammation. Kaempferol can inhibit the activation of IKK 尾, inhibit the phosphorylation of I 魏 B 伪, and prevent NF- 魏 B p65 from entering the nucleus, thus affecting the release of related inflammatory mediators.
【作者单位】：重庆医科大学药学院药理学教研室重庆市生物化学与分子药理学重点实验室;
【基金】：重庆市科委自然科学基金资助项目(CSTC2010BB5111) 重庆市教委自然科学基金资助项目(KJ100308)
【分类号】：R96

【参考文献】