星点设计-效应面法优化羟基喜树碱-粉防己碱复方脂质体的处方

发布时间：2018-03-20 05:12

本文选题：星点设计　切入点：脂质体　出处：《科学技术与工程》2017年20期 　论文类型：期刊论文

【摘要】：制备羟基喜树碱-粉防己碱复方脂质体,采用星点设计-效应面法,优选最佳的脂质体的制备工艺。制备羟基喜树碱-粉防己碱脂质体,通过单因素实验选出影响显著的三个因素胆脂比、水化温度、药脂比为自变量,包封率为因变量,根据Design-Expert6.0.5软件设计实验。通过线性回归、多元线性回归拟合后建立自变量与因变量之间的数学关系,根据数学模型绘制效应面及等高线图,从中选择最佳的实验方案。结果三项式拟合方程的r值较高,拟合度好,通过效应面选出的最佳制备工艺条件为胆脂比1∶6,水化温度51℃,药脂比1∶22,工艺验证结果与预测值的偏差分别为1.71%、1.89%通过星点设计-效应面法优化的制备复合脂质体的工艺条件准确可靠。
[Abstract]:Preparation of hydroxycamptothecin and tetrandrine compound liposomes by central composite design response surface method, the optimum preparation process of lipid body. Preparation of hydroxycamptothecin - tetrandrine liposomes by single factor experiment selected three factors significantly influence cholesterol ratio, water temperature, drug lipid ratio as the independent variable, the encapsulation rate as the dependent variable, according to the Design-Expert6.0.5 software design experiment. Through linear regression, multiple linear regression fitting after the establishment of the independent variables and the dependent mathematical relationship between variables, according to the mathematical model of response surface and contour map drawing, the best experimental scheme to choose from. Results three type fitting equation of higher R good fit through the effect of surface best preparation conditions for cholesterol ratio of 1 to 6, the water temperature of 51 DEG C, drug lipid ratio of 1: 22, process validation results deviation and predicted values were 1.71%, 1.89% by central composite design and response surface The process conditions for the preparation of composite liposomes by the method are accurate and reliable.

【作者单位】：江西中医药大学药学院;
【基金】：重点学科青年教师培养计划(2014jzzdxk036)资助
【分类号】：R943

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