阿霉素对肾上腺皮质癌细胞系SW-13克隆特性的影响

发布时间：2018-03-20 09:00

本文选题：肾上腺皮质肿瘤　切入点：克隆　出处：《实用医学杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的:研究阿霉素在体干预对肾上腺皮质癌系SW-13克隆特性的影响。方法:通过建立阿霉素干预裸鼠连续传代SW-13细胞系皮下移植瘤模型,将空白组及实验组细胞进行单细胞克隆行成试验,观察单个细胞生长曲线,并计算克隆形成率及3种克隆(全克隆、部分克隆和旁克隆)的百分比,并进行Hoechst33342试验检测细胞的耐药性。结果:SW-13细胞系经过阿霉素干预裸鼠连续传代移植瘤模型后,克隆形成率较空白对照组增加(P0.05);全克隆的百分比高于空白对照组(P0.05);经Hoechst33342试验发现经阿霉素干预肾上腺皮质癌细胞荧光减弱。结论:阿霉素在体干预提高肾上腺皮质癌细胞系SW-13的克隆形成能力及全克隆形成能力,并提高其耐药性。
[Abstract]:Objective: To study the effect of adriamycin intervention SW-13 clone characteristics of adrenal cortical carcinoma lines in vivo. Methods: through the establishment of adriamycin intervention continuous passaging in nude mice SW-13 cells subcutaneous transplantation tumor model, the blank group and the experimental group were single cell cloned into a single test, observe the cell growth curve and colony forming efficiency was calculated and 3 clone (Quan Kelong, part of cloning and cloning by) the percentage of drug resistance and Hoechst33342 test cells. Results: SW-13 cells after adriamycin intervention after continuous passaging in nude mice xenograft model, clone formation rate than the control group increased (P0.05); the percentage of total clones higher than the control group (P0.05); the the Hoechst33342 trial found that after adriamycin treatment of adrenal cortical carcinoma cells. Conclusion: the fluorescence decrease of adriamycin in body intervention to improve the adrenocortical carcinoma cell line cloned SW-13 formation Force and total cloning ability and increase its resistance.

【作者单位】：浙江中医药大学附属第二医院;广西南溪山医院;广西医科大学第一附属医院内分泌科;广西医科大学基础医学院病生教研室;
【基金】：国家自然科学基金项目(编号:81060220) 广西教育厅项目(编号:12MS061) 浙江中医药大学校级课题(编号:2014ZY17)
【分类号】：R96

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