五步蛇毒蛋白C激活物对人脐静脉血管内皮细胞组织因子表达的影响

发布时间：2018-03-20 11:03

本文选题：蛇毒液类　切入点：五步蛇毒蛋白C激活物　出处：《中国新药与临床杂志》2017年11期 　论文类型：期刊论文

【摘要】：目的观察五步蛇毒蛋白C激活物(PCA)对人脐静脉血管内皮细胞(HUVEC)的保护作用,并分析其对组织因子(TF)表达的影响。方法常规培养HUVEC,实验分为空白对照组、脂多糖(LPS)组、PCA(0.625、1.25、2.50μg·mL~(-1))组和PCA+LPS组。MTT法检测HUVEC活性,ELISA检测HUVEC培养上清中TF分泌量,免疫荧光染色检测核转录因子(NF)-κB是否被激活-核转运。结果 PCA对HUVEC活性和形态无显著影响(P0.05)。与空白对照组,LPS组细胞活性显著下降(P0.01);而LPS+PCA组细胞活性显著高于LPS组(P0.05)。LPS组TF表达量和NF-κB表达均显著高于空白对照组(P0.01);LPS+PCA组与LPS组比较TF表达量和NF-κB表达降低(P0.01)。结论 PCA可降低HUVEC分泌TF,减少LPS对HUVEC的损伤作用,其可能与抑制NF-κB通路的活化有关。
[Abstract]:Objective to observe the protective effect of PCA on human umbilical vein endothelial cells (HUVECs) and to analyze the effect of PCAs on the expression of tissue factor TF.Methods HUVECs were routinely cultured and divided into blank control group. Lipopolysaccharide (LPS) group and PCA LPS group were used to detect the activity of HUVEC and Elisa to detect TF secretion in the supernatant of HUVEC culture. Results PCA had no significant effect on the activity and morphology of HUVEC. Compared with the control group, the activity of NF-kappa B was significantly decreased, while the activity of LPS PCA group was significantly higher than that of LPS PCA group (P < 0.05), while that of LPS PCA group was significantly higher than that of LPS PCA group (P < 0.01), while that of LPS PCA group was significantly higher than that of LPS PCA group (P < 0.05). The expression of TF and NF- 魏 B in the LPS group was significantly higher than that in the control group (P 0.01) and the expression of NF- 魏 B in the LPS group was lower than that in the LPS group. Conclusion PCA can decrease the secretion of HUVEC and decrease the damage of LPS to HUVEC. It may be related to inhibiting the activation of NF- 魏 B pathway.
【作者单位】：皖南医学院病理生理学教研室;皖南医学院蛇毒研究所;
【基金】：安徽省教育厅自然科学研究基金重点项目(KJ2011A266) 活性生物大分子研究安微省重点实验室(科基[2012]126号)
【分类号】：R99

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