血小板制剂对细胞体外增殖活力影响的初步研究

发布时间：2018-03-21 05:11

本文选题：血小板　切入点：富血小板血浆　出处：《中国输血杂志》2015年11期 　论文类型：期刊论文

【摘要】：目的探讨不同血小板制剂对细胞体外增殖活力的影响,并对其作用机制进行初步研究。方法制备不同血小板制剂:新鲜机采血小板、冰冻机采血小板、富血小板凝胶上清、乏血小板血浆等,以1640培养基为对照,与MM.1S细胞(多发性骨髓瘤细胞)在相同的培养条件下进行共培养,用流式细胞仪对培养后的细胞进行细胞计数,观察细胞的增殖情况。结果 1)单独的血小板及乏血小板血浆均对MM.1S细胞有促增殖作用,且促增殖效果与样品浓度成正相关。2)新鲜机采血小板对MM.1S细胞促增殖作用在各实验组中最高,新鲜机采凝胶上清和乏血小板血浆次之;二甲基亚砜对细胞增殖有抑制作用;凝血酶对细胞增殖无明显影响。3)血小板经过冰冻处理后,机采冰冻血小板与冰冻凝胶上清对MM.1S细胞促增殖作用较新鲜机采血小板和凝胶上清减弱。但机采冰冻血小板与冰冻凝胶上清对MM.1S细胞的促增殖作用存在剂量效应,在稀释浓度40-80倍左右时,具有相对较强的促增殖影响力。结论不同血小板制剂对MM.1S细胞的促增殖作用各有不同,此研究将为制备血小板凝胶选择原辅材料提供实验依据和参考。
[Abstract]:Objective to investigate the effect of different platelet preparations on cell proliferation in vitro and its mechanism. Methods different platelets were prepared: fresh platelets, cryopreserved platelets, platelets supernatants, and platelet-rich gel supernatants. The platelet-deficient plasma was co-cultured with MM.1S cells (multiple myeloma cells) in 1640 medium, and the cells were counted by flow cytometry. Results 1) platelets and platelets alone could promote the proliferation of MM.1S cells. The effect of promoting proliferation was positively correlated with the concentration of sample. 2) the effect of fresh platelets on the proliferation of MM.1S cells was the highest, the supernatant of fresh mechanized gel and the plasma of lack of platelets were the second, and dimethyl sulfoxide inhibited the proliferation of MM.1S cells. Thrombin has no significant effect on cell proliferation. The effect of frozen platelets and supernatants on the proliferation of MM.1S cells was weaker than that of fresh platelets and supernatants of gel, but there was a dose effect on the proliferation of MM.1S cells by the supernatant of frozen platelets and supernatants of frozen gels. Conclusion the effects of different platelet preparations on the proliferation of MM.1S cells are different. This study will provide experimental basis and reference for the preparation of raw and auxiliary materials for platelet-gel selection.
【作者单位】：成都军区总医院输血科解放军成都血站;成都医学院科研实验中心;
【基金】：2011年四川省卫生厅科研课题(血站高效型血小板凝胶的制备及临床应用评价研究编号110474)
【分类号】：R96

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