光谱法研究穿琥宁与牛血清白蛋白的相互作用

发布时间：2018-03-25 07:50

本文选题：穿琥宁　切入点：相互作用　出处：《四川大学学报(自然科学版)》2017年02期

【摘要】：穿琥宁(PDS)是一种药物,被广泛用于治疗病毒性肺炎,病毒性上呼吸道感染等的消炎药,被誉为"中药抗生素".在优化的实验条件下,运用荧光和紫外光谱法,在不同的温度下研究穿琥宁与牛血清白蛋白(BSA)的相互作用及共存金属离子的影响.用Stern-Volmer,Lineweaver-Burk和双对数方程计算了速率常数(Kq),表观猝灭常数(Ksv),结合常数(KA),静态荧光猝灭缔合常数(K_(LB))和结合位点数(n).结果表明:穿琥宁能结合BSA.由于生成PDS-BSA复合物,穿琥宁对BSA的猝灭是静态猝灭机理.热力学参数表明是一个自发过程,其作用力类型主要为静电作用力.至少一个结合位点.BSA的亚螺旋域ⅡA和ⅢA是主要结合位置,离酪氨酸残基更近,无药物协同作用.穿琥宁对BSA构象产生影响.Pb~(2+)、Mn~(2+)、Ni~(2+)和Cu~(2+)对PDS与BSA结合产生竞争作用,增强药效.Cr~(3+)对穿琥宁与BSA结合产生促进作用,延长药效时间.该研究结果对揭示药物动力学问题及新的抗病毒类中草药的研发提供了理论依据.
[Abstract]:Chuanhuning (PDS) is a drug that is widely used for the treatment of viral pneumonia, viral upper respiratory tract infection and other anti-inflammatory drugs, known as the "antibiotic medicine". Under the optimized experimental conditions, using fluorescence and UV spectroscopy, study of Chuanhuning and bovine serum albumin at different temperatures (BSA) the interaction and the effect of metal ions. Stern-Volmer, Lineweaver-Burk and double logarithmic equation to calculate the rate constant (Kq), the apparent quenching constants (Ksv), the binding constant (KA), static fluorescence quenching association constant (K_ (LB)) and the number of binding sites (n). The results showed that andrographolide can with BSA. due to the formation of the PDS-BSA complex, Chuanhuning Injection on BSA quenching is static quenching mechanism. The thermodynamic parameters showed that is a spontaneous process, the force is the main type of electrostatic force. At least one of the binding sites of.BSA sub domain II and III A spiral A is the main binding site The tyrosine residues from the more recent, no synergistic effect. The influence of.Pb~ on the conformation of BSA andrographolide (2+), Mn~ (2+), Ni~ (2+) and Cu~ (2+) have competitive effects on PDS and BSA combination, enhance the efficacy of.Cr~ (3+) promotes the Chuanhuning Injection Combined with BSA extension long time. The effect of results on theoretical basis to reveal the dynamics and herbal medicine research of new antiviral in the class.

【作者单位】：曲靖师范学院化学与环境科学学院;曲靖师范学院云贵高原化学功能材料与污染治理研究中心;
【基金】：云南省教育厅科学研究基金(2015C090Y) 国家自然科学基金(21261019)
【分类号】：R961

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