重组人硫氧还蛋白对大鼠内毒素肺损伤保护作用

发布时间：2018-03-31 07:23

本文选题：重组人硫氧还蛋白　切入点：急性肺损伤　出处：《暨南大学学报(自然科学与医学版)》2015年03期

【摘要】：目的:探讨重组人硫氧还蛋白1(rh Trx-1)对新生SD大鼠急性内毒素肺损伤的保护作用.方法:制备、鉴定rh Trx-1;将新生SD大鼠分为对照组、脂多糖(LPS)实验组和LPS+rh Trx-1干预组.腹腔注射LPS(质量分数为5 mg/kg),制备急性内毒素肺损伤模型,干预组于LPS注射前30 min腹腔注射rh Trx-1(质量分数为10 mg/kg),观察注射后24 h各组新生SD大鼠的一般情况以及死亡率;重复实验,每组分别于实验后的2、8 h随机处死大鼠,提取肺组织用于病理切片观察.结果:(1)成功制备rh Trx-1;(2)LPS组大鼠表现出活动少,反应差,精神萎靡,毛发无光泽,口周发绀等全身炎症反应症状,濒死状态时表现为毛色青灰,呼吸浅表;LPS+h Trx-1干预组动物症状明显减轻;阴性对照组、LPS实验组和LPS+rh Trx-1干预组大鼠24 h死亡率分别为0%,67%、17%(醊2=14.400,P=0.001);(3)LPS组肺组织损伤严重,可见肺组织结构不完整,肺泡间隔明显增宽,肺泡腔内有红细胞、炎症细胞及浆液渗出,血管周围组织可见白细胞浸润,毛细血管有明显充血及扩张表现,随时间延长有加重趋势,LPS+rh Trx-1干预组病理改变较内毒素组明显减轻,肺泡结构尚正常,轻度肺水肿,肺泡间隔稍增宽,红细胞渗出明显减少,肺泡及肺间质中白细胞浸润减少.结论:重组人硫氧还蛋白1对急性内毒素肺损伤的新生大鼠具有保护作用.
[Abstract]:Objective: to investigate the protective effect of recombinant human thioredoxin (1(rh Trx-1) on acute endotoxin lung injury in neonatal SD rats. Lipopolysaccharide (LPS) group and LPS rh Trx-1 intervention group were injected intraperitoneally with 5 mg / kg LPS to establish acute endotoxin lung injury model. In the intervention group, rh Trx-1 was injected intraperitoneally 30 min before LPS injection (mass fraction was 10 mg / kg 路kg ~ (-1)). The general situation and mortality of neonatal SD rats were observed 24 hours after injection. Results the lung tissue was extracted from the lung tissue for pathological observation. Results the rats in the lipopolysaccharide group successfully prepared rh Trx-1 + lipopolysaccharide group showed less activity, poor response, weak spirit, dull hair, cyanosis around the mouth and other systemic inflammatory reaction symptoms. The 24 h mortality of rats in the negative control group and LPS rh Trx-1 intervention group was 0, 6717, respectively. The lung tissue injury was serious, the pulmonary tissue structure was incomplete, and the alveolar septum was obviously widened in the LPS treated group, and the mortality of the rats in the LPS rh Trx-1 intervention group was significantly higher than that in the LPS-treated group, and the mortality of the rats in the LPS-treated group was significantly higher than that in the LPS-treated group. In the alveolar cavity, red blood cells, inflammatory cells and serous exudate, leukocyte infiltration was seen in the perivascular tissues, and the capillaries showed obvious hyperemia and dilatation. The pathological changes in the LPS-Rh Trx-1 intervention group were significantly reduced as compared with that in the endotoxin group, the alveolar structure was normal, the alveolar septum was slightly wider, the alveolar septum was slightly wider, and the erythrocyte exudation was significantly decreased. Conclusion: recombinant human thioredoxin 1 has protective effect on neonatal rats with acute endotoxin lung injury.
【作者单位】：南方医科大学南方医院新生儿科;中山大学中山医学院寄生虫学教研室;
【基金】：广东省科技计划基金项目(2012B031800140)
【分类号】：R965

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