单宁酸联合顺铂增强肝癌HepG2细胞IRE1-XBP1通路的激活水平

发布时间：2018-04-01 16:14

  本文选题：单宁酸　切入点：顺铂　出处：《天然产物研究与开发》2017年11期

【摘要】：本文主要研究单宁酸(TA)联合顺铂(CDDP)对肝癌HepG2细胞内质网应激IRE1-XBP1通路的影响。用180μM单宁酸、0.9μg/m L顺铂单独用药或者联合用药处理肝癌HepG2细胞24 h或48 h后,应用流式细胞技术测定HepG2细胞的凋亡率,实时荧光定量PCR技术(q-RT-PCR)、蛋白免疫印迹(western blot)技术检测IRE1α和XBP-1分子的表达水平。MTT结果显示,单宁酸和顺铂均能显著抑制HepG2细胞的生长,且均呈剂量性依赖;二者联合用药能够显著增加HepG2细胞的生长抑制率;流式细胞术结果显示,单宁酸与顺铂联合用药能够显著抑制HepG2细胞的增殖,并诱导细胞凋亡的发生;q-RT-PCR及Western blot结果显示,单宁酸与顺铂联合用药能显著上调细胞IRE1α和XBP-1的表达水平。结果表明单宁酸能够联合顺铂增强肝癌HepG2细胞内质网应激IRE1-XBP1通路的激活水平,提示IRE1-XBP1通路可能是单宁酸和顺铂协同抗肝癌HepG2细胞的分子机制之一。
[Abstract]:In this study, we studied the effects of Tannin combined with cisplatin CDDPon on endoplasmic reticulum stress (IRE1-XBP1) pathway in hepatocellular carcinoma (HepG2) cells.The expression levels of IRE1 伪 and XBP-1 molecules were detected by quantitative real-time PCR and Western blot. The results showed that both tannic acid and cisplatin could significantly inhibit the growth of HepG2 cells in a dose-dependent manner.The results of flow cytometry showed that the combination of tannic acid and cisplatin could significantly inhibit the proliferation of HepG2 cells, and induce apoptosis of HepG2 cells by q-RT-PCR and Western blot.Tannic acid combined with cisplatin could significantly up-regulate the expression of IRE1 伪 and XBP-1.The results showed that tannic acid combined with cisplatin could enhance the activation level of endoplasmic reticulum stress IRE1-XBP1 pathway in HepG2 cells, suggesting that the IRE1-XBP1 pathway might be one of the molecular mechanisms of synergistic effect of tannic acid and cisplatin on hepatoma HepG2 cells.
【作者单位】： 遵义医学院口腔学院;遵义医学院医学与生物学研究中心贵州省普通高等学校口腔疾病研究特色重点实验室;遵义医学院医学遗传学教研室;
【基金】：贵州省教育厅特色药用资源研发创新团队(2013-15) 贵州省科技厅资助(2014-7548) 遵义医学院硕士启动基金(F-841);遵义医学院硕士启动基金(F-827)
【分类号】：R96
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本文编号：1696275