Heparosan细胞入胞途径及其胞内分布

发布时间：2018-04-01 17:05

本文选题：肝素前体　切入点：入胞途径　出处：《药学学报》2017年03期

【摘要】：本文对肝素前体(heparosan)的细胞摄取途径及其进入细胞后的分布情况进行了研究。通过胞吞途径抑制及细胞探针定位实验发现,MCF-7肿瘤细胞较COS7正常细胞对heparosan的摄取效率更高,具有选择性。Heparosan在MCF-7肿瘤细胞和COS7正常细胞中的内吞过程均为能量依赖。Heparosan主要通过小窝蛋白和巨胞饮介导的内吞进入MCF-7肿瘤细胞,并且主要分布于溶酶体中。
[Abstract]:In this paper, we studied the uptake pathway of heparosan and its distribution after entering the cells. Through the inhibition of cytoplasmic pathway and the localization of cell probe, we found that the uptake efficiency of heparosan in MCF-7 tumor cells was higher than that in normal COS7 cells. The endocytosis process of selective .Heparosan in both MCF-7 tumor cells and COS7 normal cells is energy-dependent. Heparosan enters MCF-7 tumor cells mainly through fossa protein and macrocytosine mediated endocytosis and mainly distributes in lysosomes.
【作者单位】：江南大学药学院;
【基金】：国家自然科学基金资助项目(51303068,21574059,21306066) 安徽大学现代生物制造协同创新中心开放课题资助项目(BM2015008)
【分类号】：R96

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