气相色谱-质谱法测定大鼠血浆中尼古丁及其药物代谢动力学研究

发布时间：2018-04-01 23:09

  本文选题：气相色谱-质谱法　切入点：尼古丁　出处：《理化检验(化学分册)》2015年07期

【摘要】：采用气相色谱-质谱法测定大鼠血浆中的尼古丁。血浆样品采用蛋白沉淀法净化,采用DB-Wax毛细管色谱柱分离,质谱中选择电子轰击离子源和选择离子监测模式,以茴香脑为内标进行定量。并采用PKSlover 2.0软件计算药代动力学参数。尼古丁的线性范围为0.10~2.0mg·L-1,检出限为4.59μg·L-1。对空白样品进行加标回收试验,回收率在85.0%~90.1%之间,相对标准偏差(n=6)小于3.0%。应用此方法考察了灌胃给药的大鼠血浆样品中尼古丁代谢动力学特点,主要动力学参数AUC0-t,AUC0-∞,Tmax和ρmax分别为8.570mg·L-1·h-1,10.49mg·L-1·h-1,1.0h,998.9μg·L-1,结果呈现药物代谢动力学过程。
[Abstract]:Nicotine in rat plasma was determined by gas chromatography-mass spectrometry. Plasma samples were purified by protein precipitation and separated by DB-Wax capillary column. Electron bombardment ion source and ion monitoring mode were selected in mass spectrometry. PKSlover 2.0 software was used to calculate the pharmacokinetic parameters. The linear range of nicotine was 0.10~2.0mg L -1 and the detection limit was 4.59 渭 g L -1. The recovery rate of the blank sample was 85.0%, and the recovery rate was 90.1%. The pharmacokinetic characteristics of nicotine in plasma samples of rats were studied by using this method. The main kinetic parameters were AUC0-t0 ~ (- 鈭，

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