丙戊酸钠血药浓度测定衍生因素分析

发布时间：2018-04-04 09:00

  本文选题：丙戊酸钠　切入点：血药浓度　出处：《中国医院药学杂志》2017年13期

【摘要】：目的:分析丙戊酸钠血药浓度测定衍生时的影响因素,保证临床测定的准确性。方法:丙戊酸钠血药浓度用高效液相色谱(HPLC)法测定。血浆样品用2-溴-对硝基苯乙酮为衍生试剂,以丙戊酸钠衍生物产率为指标,用单因素考察法考察衍生试剂浓度、三乙胺体积分数、反应温度、反应时间对丙戊酸钠衍生产率的影响。结果:衍生试剂2-溴-对硝基苯乙酮浓度为1.5~2.0mg·mL~(-1),催化剂三乙胺体积分数为20%,衍生温度和衍生时间分别为60℃,10 min时,丙戊酸衍生完全,血药浓度在10~200μg·mL~(-1)范围内,线性较好(R~2=0.999 5),回收率为95.00%~100.59%,日内与日间RSD≤5%,符合《中国药典》中对生物样本药物浓度检测的规定。结论:影响丙戊酸血药浓度测定的因素较多,如衍生试剂浓度和三乙胺体积分数、衍生时间、衍生温度等。在日常血药浓度监测过程中,我们应注意这些差异,尽量将误差控制在允许范围之内,保证丙戊酸钠治疗药物监测结果的准确性。
[Abstract]:Objective: to analyze the factors influencing the determination of valproate concentration in blood and to ensure the accuracy of clinical determination.Methods: the concentration of sodium valproate in blood was determined by high performance liquid chromatography (HPLC).The concentration of derivative reagent, the volume fraction of triethylamine, the reaction temperature and the yield of sodium valproate derivatives were investigated by single factor method with 2-bromo-p-nitroacetophenone as derivative reagent and sodium valproate derivative yield as index.The effect of reaction time on the productivity of sodium valproate.Results: when the concentration of 2-bromo-p-nitroacetophenone was 1.5~2.0mg mLL-1, the volume fraction of triethylamine was 20, and the derivatization temperature and time were 60 鈩，

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