HPLC法同时测定注射用三重霉素中普鲁卡因、二苄基乙二胺和青霉素的含量
发布时间:2018-04-06 00:09
本文选题:注射用三重霉素 切入点:普鲁卡因 出处:《中国药房》2017年03期
【摘要】:目的:建立同时测定注射用三重霉素中普鲁卡因、二苄基乙二胺、青霉素含量的方法。方法:采用高效液相色谱法。色谱柱为Sino Chrom C_(18),流动相为0.21 mol/L磷酸二氢钾溶液(含0.16%的三乙胺,氢氧化钾溶液调pH至5.6)-乙腈(81∶19,V/V),流速为1.0 mL/min,检测波长为215 nm,柱温为25℃,进样量为10μL。结果:普鲁卡因、二苄基乙二胺、青霉素检测质量浓度线性范围分别为0.011~0.64 mg/mL(r=0.999 9)、0.013~0.80 mg/mL(r=0.999 9)、0.075~4.50 mg/mL(r=0.999 9);精密度、稳定性、重复性试验的RSD0.70%;加样回收率分别为98.42%~101.51%(RSD=0.90%,n=9)、98.28%~101.50%(RSD=0.90%,n=9)、99.03%~101.11%(RSD=0.66%,n=9)。结论:该方法简便、准确,可用于注射用三重霉素中普鲁卡因、二苄基乙二胺和青霉素含量的同时测定。
[Abstract]:Objective : To establish a method for simultaneous determination of proparacaine , dibenzyl ethylenediamine and penicillin in injection for injection . The chromatographic column was Sino Chrom C _ ( 18 ) , the mobile phase was 0.21 mol / L potassium dihydrogen phosphate solution ( containing 0.16 % triethylamine , potassium hydroxide solution adjusted to 5.6 ) - acetonitrile ( 81 : 19 , V / V ) , the flow rate was 1.0 mL / min , the detection wavelength was 215 nm , the column temperature was 25 鈩,
本文编号:1717084
本文链接:https://www.wllwen.com/yixuelunwen/yiyaoxuelunwen/1717084.html
最近更新
教材专著