瑞舒伐他汀对同型半胱氨酸损伤人脐静脉内皮细胞血管细胞间黏附分子-1表达的影响

发布时间：2018-04-08 18:07

本文选题：瑞舒伐他汀　切入点：内皮细胞　出处：《广东医学》2015年11期

【摘要】：目的探讨瑞舒伐他汀(Rosu)对同型半胱氨酸(Hcy)损伤人脐静脉内皮细胞(HUVECs)血管细胞间黏附分子-1(VCAM-1)表达的影响。方法原代培养HUVECs,2~3代进行试验,MTT实验设立空白对照组、Hcy(0.2~1.2 mmol/L)组、Rosu(0.5、1.0、2.0和5.0 mmol/L)组,实验组:Rosu 1.0 mmol/L和2.0 mmol/L进行预处理细胞,2 h后加入0.6 mmol/L浓度的Hcy损伤HUVECs,Real time PCR法测定VCAM-1 mRNA表达,Western blot法测定细胞VCAM-1蛋白含量;Rosu 2.0 mmol/L+甲羟戊酸(Mev)100μmol/L预处理2 h后再加入0.6mmol/L浓度的Hcy以了解Mev对VCAM-1蛋白表达的影响。结果 Hcy组VCAM-1蛋白和mRNA表达较空白对照组明显增强(P0.05);Rosu组VCAM-1蛋白和mRNA表达较Hcy组明显减弱(P0.05),且Mev不能阻断Rosu的这种作用。结论 Rosu可通过抑制HUVECs表达VCAM-1产生抗炎作用,且这种作用独立于其调脂作用。
[Abstract]:Objective to investigate the effect of rosuvastatin on the expression of intercellular adhesion molecule-1 (VCAM-1) in human umbilical vein endothelial cells (HUVECs) injured by homocysteine (Hcy).Methods the primary culture of HUVECs2 ~ 2 ~ (2 +) was carried out by MTT assay. The control group was established as the control group, the control group was the control group, the control group was the control group, the control group was the control group, the control group was the control group, the control group was the control group, the control group was the control group, the control group was the control group.The cells of the experimental group were pretreated with 1. 0 mmol/L and 2. 0 mmol/L for 2 h, then the cells were pretreated with 0. 6 mmol/L concentration of Hcy for 2 h. The expression of VCAM-1 mRNA was measured by real time PCR method. Western blot method was used to determine the VCAM-1 protein content of the cells. Rosu 2. 0 mmol/L Mevedol 100 渭 mol/L was pretreated for 2 h, then 0.6mmol/L was added.Degree of Hcy to understand the effect of Mev on the expression of VCAM-1 protein.Results the expression of VCAM-1 protein and mRNA in Hcy group was significantly higher than that in control group. The expression of VCAM-1 protein and mRNA in Hcy group was significantly lower than that in Hcy group, and Mev could not block this effect of Rosu.Conclusion Rosu can produce anti-inflammatory effect by inhibiting HUVECs expression of VCAM-1, and this effect is independent of its lipid regulation.
【作者单位】：中山大学附属第三医院心内科;中山大学附属第三医院放射科;
【分类号】：R96

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