多柔比星对心肌细胞的损伤作用及对microRNA的影响

发布时间：2018-04-10 08:26

本文选题：多柔比星　切入点：心肌毒性　出处：《安徽医科大学学报》2017年01期

【摘要】：目的探讨多柔比星对原代乳鼠心肌细胞的损伤作用及其对微小RNA(miRNA)表达的影响。方法原代培养SD大鼠乳鼠心肌细胞,随机分为6组:对照组,不同浓度多柔比星(0.125、0.25、0.5、1、2μmol/L)处理组。对照组细胞正常培养,处理组细胞使用终浓度分别为0.125、0.25、0.5、1、2μmol/L的多柔比星处理24 h。倒置显微镜下观察心肌细胞搏动频率,CCK-8法检测细胞活力,微板法检测细胞培养液中乳酸脱氢酶(LDH)活性,荧光定量RT-PCR(qRTPCR)法检测心肌细胞miR-133a-5p、miR-133b-5p、miR-6216和miR-30e-5p表达水平。结果与对照组相比,多柔比星减慢心肌细胞搏动频率,降低细胞活力,升高LDH活性(P0.05)。qRT-PCR结果显示,与对照组相比,多柔比星1μmol/L处理组心肌细胞miR-133b-5p、miR-6216和miR-30e-5p表达显著上调,miR-133a-5p表达显著下调。结论多柔比星对原代乳鼠心肌细胞具有损伤作用,其机制可能与调控miR-133a-5p、miR-133b-5p、miR-6216和miR-30e-5p等miRNA表达有关。
[Abstract]:Objective to investigate the damage effect of doxorubicin on primary neonatal rat cardiomyocytes and the effect of doxorubicin on the expression of miRNAs.Methods Primary cultured neonatal SD rat cardiomyocytes were randomly divided into 6 groups: control group, treated with different concentrations of doxorubicin 0.125 (0.25) and 0.5 渭 mol / L (1 渭 mol / L).In the control group, the cells were treated with doxorubicin for 24 h with the final concentration of 0.125 渭 mol/L of 0.25 渭 mol/L and 0.5 渭 mol/L of doxorubicin, respectively.The activity of lactate dehydrogenase (LDH) was detected by microplate method, and the expression of miR-133a-5pnmiR-133b-5pnmiR-6216 and miR-30e-5p in cardiomyocytes was detected by fluorescence quantitative quantitative method.Results compared with the control group, doxorubicin slowed down the beating frequency of cardiomyocytes, decreased cell viability and increased the activity of LDH.The expression of miR-133b-5pmmiR-6216 and miR-30e-5p in cardiac myocytes treated with doxorubicin 1 渭 mol/L significantly up-regulated the expression of miR-133a-5p.Conclusion doxorubicin can injure primary neonatal rat cardiomyocytes by regulating the expression of miR-133a-5pnmiR-133b-5pmmiR-6216 and miR-30e-5p.
【作者单位】：安徽医科大学第二附属医院麻醉科;
【基金】：国家自然科学基金青年科学基金项目(编号:81200171) 安徽高校省级自然科学研究项目(编号:KJ2014ZD16)
【分类号】：R965

【相似文献】