厄洛替尼引起皮肤不良反应的小鼠模型建立

发布时间：2018-04-12 11:56

  本文选题：厄洛替尼 + 皮疹　； 参考：《中国比较医学杂志》2017年01期

【摘要】：目的厄洛替尼(商品名:特罗凯)是治疗非小细胞肺癌的一种新型靶向药物,近些年来临床应用甚广,但副作用较多,其中以皮疹最为多见,且最令患者难以接受。本研究旨在观察应用特罗凯前后小鼠在表皮、病理、免疫组化等方面的改变,复制特罗凯所致皮疹的动物模型,为临床外用药物治疗皮疹提供模型。方法使用BALB/c雌性小鼠20只,随机分成4组。实验组(Ⅱ、Ⅲ、Ⅳ组)用浓度为10 g/L的特罗凯溶液按100 mg/kg灌胃,对照组(Ⅰ组)以等体积去离子水灌胃,每天1次。于给药前24 h在小鼠头颈、背、腰处脱毛,实验结束后剪取颈部、背部、腰部皮肤并观察实验组、对照组小鼠在肉眼皮肤、病理切片、免疫组化等方面的改变。结果 (1)四组小鼠之间在毛发再生天数、毛发完全再生天数、脱屑时间、出现皮疹时间、毛孔扩张数等5方面的差异存在统计学意义(P0.01或P0.05);(2)Ki67:四组间差异无统计学意义(P0.05)。结论 (1)此次实验印证了许多研究者关于"EGFRIs导致的皮疹为一种炎症反应"的观点;(2)该皮疹模型可靠实用,具有可重复性,适用于"EGFRIs所致皮疹动物模型"的大批建立,可推广以供临床、试验、研究所用。
[Abstract]:Objective erlotinib (trade name: trokei) is a new target drug for the treatment of non-small cell lung cancer (NSCLC). In recent years, it is widely used in clinical practice, but has many side effects, among which rash is the most common and most difficult for patients to accept.The purpose of this study was to observe the changes of epidermis, pathology and immunohistochemistry in mice before and after troke-induced skin rash, and to establish the animal model of the skin rash induced by troke-induced skin rash, and to provide a model for the treatment of skin rash by external medicine.Methods Twenty BALB/c female mice were randomly divided into 4 groups.The experimental group (group 鈪，

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