2-羟丙基-β环糊精-过氧化苯甲酰包合物与小牛胸腺DNA的相互作用

发布时间：2018-04-12 13:12

本文选题：-羟丙基-β环糊精 + 过氧化苯甲酰　；参考：《南昌大学学报(理科版)》2017年02期

【摘要】：在生理酸度条件下(pH 7.4),运用多种光谱分析方法研究了2-羟丙基-β环糊精(Hp-βCD)-过氧化苯甲酰(BPO)包合物与小牛胸腺DNA(ctDNA)的相互作用。结果表明,Hp-βCD与BPO形成1鐩1的包合物,两者的包合常数为2.40×10~4 L·mol~(-1)。计算出的热力学参数表明,氢键和范德华力是Hp-βCD-BPO包合物与ctDNA相互作用的主要驱动力。Hp-βCD-BPO包合物存在下,ctDNA的熔点和粘度无明显变化,KI荧光猝灭效应和Na3PO4的存在使Hp-βCD-BPO-ctDNA复合物的荧光强度发生改变,表明Hp-βCD-BPO包合物与ctDNA主要通过沟槽和静电方式结合。红外光谱(FT-IR)和圆二色谱(CD)分析结果显示,Hp-βCD-BPO包合物主要与ctDNA的鸟嘌呤(G碱基)发生特异性结合,并引起ctDNA的B构象向A构象转变。
[Abstract]:The interaction between the inclusion compound of 2-hydroxypropyl- 尾 cyclodextrin (Hp- 尾 CDP- BPO-benzoyl peroxide) and calf thymus DNA (ctDNA) was studied by using various spectral methods at pH 7.4 under physiological acidity.The results show that Hp- 尾 CD and BPO form a inclusion complex of 1 ~ 1, and their inclusion constants are 2.40 脳 10 ~ (4) L / mol ~ (-1) ~ (-1).The calculated thermodynamic parameters show that,Hydrogen bond and van der Waals force are the main driving forces of the interaction between Hp- 尾 CD-BPO inclusion complex and ctDNA. The melting point and viscosity of ctDNA in the presence of Hp- 尾 CD-BPO inclusion complex have no obvious change. The fluorescence intensity of Hp- 尾 CD-BPO-ctDNA complex is changed by the fluorescence quenching effect of Ki and the existence of Na3PO4.The results show that the Hp- 尾 CD-BPO inclusion compound binds to ctDNA mainly through grooves and electrostatics.The results of FT-IR and CD CD analysis showed that the inclusion complex of Hp- 尾 CD-BPO mainly binds specifically to the guanine G base of ctDNA, and leads to the transformation of B conformation to conformation A of ctDNA.
【作者单位】：南昌大学食品科学与技术国家重点实验室;
【基金】：国家自然科学基金资助项目(3146042,221167013) 江西省自然科学基金资助项目(20143ACB20006) 食品科学与技术国家重点实验室目标导向课题(SKLF-ZZA-201612)
【分类号】：O657.3;R96

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