西曲瑞克和曲普瑞林对小鼠免疫效应的对比实验研究

发布时间：2018-04-13 03:09

本文选题：西曲瑞克 + 曲普瑞林　；参考：《免疫学杂志》2015年07期

【摘要】：目的探讨促性腺激素释放激素拮抗剂和激动剂抗原主动免疫对小鼠免疫效果、生长与器官发育和生殖内分泌的作用。方法 105只昆明雌鼠随机均分为西曲瑞克组(CET)、曲普瑞林组(TRI)和对照组(CG),在CET-1、CET-2及CET-3小组分别皮下注射曲普瑞林抗原0.1、0.2及0.4 ml,TRI-1、TRI-2及TRI-3小组分别皮下注射西曲瑞克抗原0.1、0.2及0.4 ml,CG组注射生理盐水0.2 ml,各组均连续注射7 d。精确测定体质量,于0、21及35 d从各小组分别抓取5只小鼠眼眶后静脉丛采血,分离血清;同时断颈处死,无菌采集心脏、肝脏、脾脏、肾脏、子宫及卵巢,分别称质量,计算日增质量和内脏指数。ELISA试剂盒测定血清Gn RH抗体、LH和Gn RH质量浓度。结果实验期间CET-3组体质量大于CG组(P0.01),CET-1和CET-2组小鼠体质量在12 d和21 d时小于CG组(P0.05);8 d后TRI-1小组体质量低于CG组(P0.05);TRI-2和TRI-3组分别于16 d和21 d降至低谷。CET-3和TRI-1组肝脏与脾脏质量均明显大于CG(P0.05),35 d时CET-3、TRI-1、TRI-2和TRI-3组子宫质量分别减小31.63%、21.55%、63.1%和61.71%;CET-1和CET-2组子宫质量比CG增加40.78%和11.94%。CET和TRI的血清Gn RH抗体浓度高于CG,且TRI组的高于CET组,尤其是TRI-3组更明显(P0.01)。21 d各实验组Gn RH质量浓度均低于CG,TRI-3组显著低于CG(P0.05)。至35 d,CET-1、CET-2、TRI-2和TRI-3组Gn RH质量浓度显著低于CG(P0.05),CET-3组则高于CG。CET-3和TRI-3组血清LH质量浓度显著低于CG。结论西曲瑞克和曲普瑞林主动免疫能剂量依赖性地促进机体产生Gn RH抗体,免疫抑制Gn RH和LH合成与分泌,低剂量免疫注射初期抑制小鼠生长,高剂量后期明显促进肝脏和脾脏的生长发育、抑制子宫发育,且曲普瑞林的作用更加明显。
[Abstract]:Objective to investigate the effects of active immunization of gonadotropin releasing hormone antagonists and agonists on mice's immune response, growth, organ development and reproductive endocrine.Methods one hundred and five Kunming female rats were randomly divided into two groups: citrork group, tripline group and control group. The mice were subcutaneously injected with triplatin antigen 0.1C 0.2 and 0.4 ml TRI-1 TRI-2 and TRI-3 group respectively.And 0.4 ml CG group were injected with 0.2 ml saline for 7 days.The blood samples were collected from the posterior orbital venous plexus of 5 mice on the 21st and 35th day, and the blood serum was isolated from the posterior orbital venous plexus of the mice. At the same time, the mice were killed by cervical amputation, and the heart, liver, spleen, kidney, uterus and ovary were collected and the weight was measured.Daily gain and visceral index. Elisa kit were used to determine serum GnRH antibody LH and GnRH concentration.The uterine weight of CET-3 / TRI-1TRI-2 group and TRI-3 group decreased by 31.63 ~ 21.5551% and 61.71%, respectively, and the serum GnRH antibody concentration of 11.94%.CET and TRI was higher than that of CG, and the serum GnRH antibody concentration of TRI group was higher than that of CET group.Especially in TRI-3 group, the concentration of Gn RH in each experimental group was significantly lower than that in CGT TRI-3 group.The concentration of GnRH in CET-1 CET-2 TRI-2 and TRI-3 group was significantly lower than that in CGP 0.05 CET-3 group, but higher than that in CG.CET-3 and TRI-3 group, and the serum LH concentration in TRI-3 group was significantly lower than that in CG.CET-3 and TRI-3 group.Conclusion active immunization with citrork and triplatin can promote the production of GnRH antibody in a dose-dependent manner, immunosuppresses the synthesis and secretion of GnRH and LH, and inhibits the growth of mice at the initial stage of low dose immunization.At the late stage of high dose, the growth and development of liver and spleen were obviously promoted, and the uterine development was inhibited, and the effect of triplatin was more obvious.
【作者单位】：西北民族大学生命科学与工程学院;西北民族大学医学院;甘肃省人民医院神经内科;
【基金】：国家自然科学基金(31460684,31060350)
【分类号】：R965

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