布比卡因对人肺上皮细胞短路电流影响的研究

发布时间：2018-04-13 14:34

本文选题：布比卡因 + 短路电流　；参考：《中国药理学通报》2017年02期

【摘要】：目的布比卡因是长效酰胺类局麻药,本研究旨在观察布比卡因对人肺上皮细胞短路电流的影响,并探讨可能的机制。方法应用尤斯灌流室装置测定H441单层细胞的短路电流。由总电流减去阿米洛利抑制后的电流算得阿米洛利敏感性电流,以用药前H441单层细胞的阿米洛利敏感性电流初始值为100%对照。用100μmol·L~(-1)布比卡因处理H441细胞,在0、15、30、60 min 4个时间点提取蛋白用于Western blot,研究布比卡因对ERK1/2蛋白磷酸化的影响。结果布比卡因能够剂量依赖性抑制H441单层细胞的短路电流,此电流可被阿米洛利抑制;Western blot结果显示,布比卡因能够促进ERK1/2蛋白磷酸化。结论布比卡因通过抑制人肺上皮细胞阿米洛利敏感性电流而降低肺泡上皮离子转运,其机制可能与其促进ERK1/2蛋白磷酸化有关。临床上对伴有肺部疾患的病人应用布比卡因时应考虑其可能对肺泡上皮液体清除的影响。
[Abstract]:Objective Bupivacaine is a long-acting amide local anesthetic. The purpose of this study was to observe the effect of bupivacaine on short circuit current in human lung epithelial cells and to explore the possible mechanism.Methods the short-circuit current of H 441 monolayer cells was measured by using Yus perfusion chamber device.The total current was subtracted from the amilolipid inhibition current to calculate the amiloline-sensitive current, and the initial value of amiloride sensitivity current in H441 monolayer cells was 100% control.H441 cells were treated with Bupivacaine (100 渭 mol). The protein extracted from H441 cells was used for Western blotts at four time points of 0 ~ 1530 ~ (60 min) to study the effect of bupivacaine on the phosphorylation of ERK1/2 protein.Results Bupivacaine could inhibit the short circuit current of H441 monolayer cells in a dose-dependent manner. The results showed that bupivacaine could promote the phosphorylation of ERK1/2 protein.Conclusion Bupivacaine can reduce the ionic transport of alveolar epithelium by inhibiting the sensitive current of amiolol in human lung epithelial cells, which may be related to its promotion of phosphorylation of ERK1/2 protein.The effect of bupivacaine on alveolar epithelial fluid clearance should be considered in patients with pulmonary diseases.
【作者单位】：中国医科大学附属第一医院麻醉科;武警辽宁省总队医院胸外科;中国医科大学代谢病分子机制与药物研究所;
【基金】：国家自然科学基金资助项目(No81270098) 辽宁省自然科学基金资助项目(No2014021093)
【分类号】：R96

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