ICP-AES法考察硫糖铝对SD大鼠胃溃疡粘膜的靶向粘附

发布时间：2018-04-14 02:18

本文选题：硫酸化二糖的酸酯铝盐复合物 + 硫糖铝　；参考：《药物分析杂志》2016年12期

【摘要】：目的:建立硫糖铝中铝离子(Al~(3+))在大鼠胃组织残留量的测定方法,考察硫糖铝对胃溃疡粘膜的靶向粘附作用。方法:采用干法灰化消解胃组织样品,电感耦合等离子体-原子发射光谱法(ICP-AES)测定给药后不同时间点大鼠胃组织中Al~(3+)的残留量。ICP-AES检测波长为396.153 nm,检测器为电感耦合阵列检测器,载气为氩气。结果:干法灰化大鼠胃溃疡组织,样品消解完全,可满足ICP-AES的检测要求;在选定的最佳条件下用ICP-AES检测Al~(3+)的检出限为8 ng·m L~(-1),样品间Al~(3+)残留量相对偏差小于13.4%,回收率在96.0%~99.5%之间;给药0.5~4 h时间段内,溃疡胃组织中的Al~(3+)残留率为正常胃组织的2倍以上;给药6 h后,正常胃组织中Al~(3+)残留率显著下降,仅为0.84%,而溃疡胃组织在给药16 h后仍高达29%。结论:ICP-AES测定大鼠胃组织中Al~(3+)的残留量准确可行;硫糖铝对胃溃疡粘膜的粘附性显著高于正常胃粘膜,具有良好的靶向性。
[Abstract]:Aim: to establish a method for the determination of the residual amount of aluminum ionomer in the gastric tissue of rats, and to investigate the targeted adhesion of sucralfate to gastric ulcer mucosa.Methods: gastric tissue samples were digested by dry ashing.Inductively coupled plasma-atomic emission spectrometry (ICP-AES-) was used to determine the residual amount of Al~(3 in rat stomach at different time points. The detection wavelength of ICP-AES was 396.153 nm, the detector was inductively coupled array detector, and the carrier gas was argon.Results: the samples of gastric ulcer tissue were completely digested by dry ashing method, which could meet the requirements of ICP-AES detection.Under the optimum conditions, the detection limit of Al~(3 by ICP-AES was 8 ng mL / L ~ (-1), the relative deviation of Al~(3) between samples was less than 13.4%, the recovery rate was between 96.0% and 99.5%.The residual rate of Al~(3 in gastric ulcer tissue was more than 2 times as high as that in normal gastric tissue, and the residual rate of Al~(3 in normal gastric tissue decreased significantly after 6 h of administration, only 0.84%, while the gastric tissue of ulcer was still up to 29% after 16 hours of administration.Conclusion the residual amount of Al~(3 in gastric tissue of rats is accurate and feasible, and the adhesion of sucralfate to gastric ulcer mucosa is significantly higher than that of normal gastric mucosa.
【作者单位】：重庆医药高等专科学校;重庆市食品药品检验检测研究院;
【分类号】：R965

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